CAS 77-86-1 Trimethylolpropane Tris Aminoethane Tromethamine Aminobutanol THAM

Trimethylolpropane tris(2-methyl-1-aziridinepropionate) CAS 64265-57-2 Aminoethane Tromethamine Aminobutanol THAM

Synthesis of Biological Buffer TRIS
TRIS stands for Tris(hydroxymethyl)aminomethane, CAS number is 77-86-1. It is white crystalline powder with good water solubility. It is soluble in ethanol, ethyl acetate, benzene and water. And it is corrosive to copper and aluminum. When used as a biological buffer and a reference material for acid titration, the purity requirements are relatively high.

However, at present, the preparation of benchmark tris is not particularly perfect. The commonly used method is to dissolve technical grade trishydroxymethylaminomethane in distilled water, and obtain benchmark grade trishydroxymethylaminomethane after multiple recrystallizations. This method has disadvantages such as complicated process, small output, poor uniformity, and high cost when producing kilogram-level batches. In addition, the high temperature during the concentration process causes the material to change color or decompose, resulting in a lower product qualification rate.

Synthetic process of tris(hydroxymethyl)aminomethane:

1. The preparation method of tris: adding tris to the methanol aqueous solution, heating to 50-70°C and stirring to dissolve, wherein the mass volume ratio of the tris to the methanol aqueous solution is in g /ml is 8:3-7, the methanol aqueous solution is prepared by mixing pure water and methanol in a volume ratio of 2:3; adding charcoal activated carbon to the solution, wherein the charcoal activated carbon and trimethylolmethane The weight ratio is 0.5-2:100, keep at 45-55°C for 20-40 minutes, then filter while hot, and collect the filtrate; concentrate the filtrate under reduced pressure at 70-80°C until crystallization occurs, and let it cool down; After the crystals are separated by suction filtration, they are rinsed with absolute ethanol and dried at 40-60°C for 3-5 hours.

2. The synthesis method of tris(hydroxymethyl)aminomethane: it uses nitromethane and excess paraformaldehyde as the main raw materials, and it is condensed to form tris(hydroxymethyl)nitromethane at a certain temperature; then at a certain temperature, pressure, and solvent With catalyst, through hydrogenation reduction reaction, crystallization and recrystallization are made into trishydroxymethylaminomethane.

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