Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid 99% Purity

Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid 99% Purity

Good’s buffer PIPES quality manufacturer-Desheng

Keywords: Good’s buffer, PIPES, piperazine-1,4-diethanesulfonic acid

The full name of PIPES is piperazine-1,4-diethanesulfonic acid, a substance that can maintain the pH of the solution within a certain range. When a certain amount of acid and alkali is added to some solutions, it has the effect of hindering the pH change of the solution, which is called a buffering biological buffer. Such a solution is called a buffer solution. PIPES is an important member of Good’s buffer. Good’s buffer is a buffer system dedicated to life science research, which does not participate in and does not interfere with biochemical processes.

Structure diagram of piperazine-1,4-diethanesulfonic acid (PIPES)

The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine). It cannot form stable complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. Since PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.

1-4-Piperazine Diethylsulfonic Acid Pipes Biological Buffer

Pipes, Chinese name 1-4-piperazine diethylsulfonic acid, is an amphoteric buffer solution, pH buffer range is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. It has a good buffer capacity in the range of pH 7.2-7.4.

In 1960, one of the series of ethanesulfonic acid buffer reagents developed by good's et al. Has some important characteristics common to good's buffer solutions, including:

1) Low UV (λ≥ 260 nm) absorbance;

2) The solubility in water is the highest, but the solubility in other solvents is the lowest;

3) PKa is located in the neutral range and is not easily affected by temperature;

4) The pH value is stable;

5) The interference reaction is small and the enzyme or chemistry is stable;

6) It is easy to synthesize.

The effective buffer range of pipes was pH 6.1-7.5, PKA = 6.8 (25 ℃). Because its PKA is close to physiological pH, it is suitable for cell culture. In addition, in animal and plant histochemistry, pipes can also be used as a buffer reagent to minimize the lipid damage and experimental illusion caused by glutaraldehyde fixation.

Different from buffers containing bis (2-hydroxyethyl) amino groups (such as bis Tris, bicine), pipes can not form stable complexes with most metal ions, so it is suitable for buffers containing metal ions. According to previous studies, PIPES can be used to purify tubulin by using cellulose phosphate chromatography, and to purify recombinant GTP binding protein ARF1 and ARF2 by gel filtration as a buffer to crystallize ketoenzyme from E. coli.

Due to the formation of free radicals, pipe is not suitable for redox system. In cation exchange chromatography, low concentration of pipes buffer should be used because of its relatively high ionic strength and concentration dependent pKa value.

The pKa value of pipes is close to physiological pH, and it can not be absorbed when passing through cell membrane, and it can pass through ultraviolet. In the study of lactate oxidase method for the determination of lactic acid in plasma, it was found that the stability of pipe at 37 ℃ for 172 hours was better than that of Tris for 72 hours.

The results showed that the PI staining solution prepared with pipes buffer could be used to analyze the cell proliferation and DNA ploidy of clinical tumor, and the 4% paraformaldehyde prepared with 50 mmol / L pipes buffer could be used for immunofluorescence labeling.

When adenosine diphosphate (ADP) binds to flabby fan-shaped myofibroblasts, pipe can be used as a buffer to locate the ultrastructure of uric acid oxidase in rat liver peroxisome, and can also induce the polymerization of purified tubulin.

Pipes is widely used in molecular biology, pharmacology and other scientific research. It is strictly prohibited to be used in human body. The product developed and produced by Desheng can be used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit. But we need to pay attention to the following points when using it:

1. The solution used in the experiment should be prepared with pure water, and the container should be cleaned with pure water more than three times before use.

2. Pipes buffer is sensitive to light and easy to decompose, so it should be packed in brown container.

3. Each solution shall be marked with name, specification, concentration and preparation date.

4. It is forbidden to contact the solution directly with the skin, so as to avoid the body injury caused by toxic substances.

5. Avoid the solution deterioration caused by the drastic change of temperature, and take it as you use it, so as to avoid the error of analysis results.

Because each cell has different sensitivity to the solution, it is necessary to determine the concentration range of the solution to be screened before screening cells (too high concentration will kill all cells, too low concentration will not be toxic, both of which can not play a screening role).

Preparation method of PIPES solution (about 1-2L) with different pH value

1. With 0.1M solution (A)

(1) NaOH/deionized water=4g/400ml …①

(2) "①+PIPES 30.237g"...②

(3) ②+Deionized water (about 600ml)=1,000ml

2. With 0.1M NaOH solution (B): NaOH 4g/ deionized water 1,000ml

3.pH 5.6 1,000ml(A)+0ml(B) (A):(B)=5:0

pH 6.4 1,000ml(A)+200ml(B) (A):(B)=5:1

pH 6.8 1,000ml(A)+400ml(B) (A):(B)=5:2

pH 7.2 1,000ml(A)+600ml(B) (A):(B)=5:3

pH 7.7 1,000ml(A)+800ml(B) (A):(B)=5:4