Liquid VTM Virus Transport Media For Nucleic Acid Detection COVID 2019 Sampling Tubes.

Liquid VTM Virus Transport Media for Nucleic Acid Detection for COVID 2019 etc.

The Virus Transport Media is mainly the component added in the virus sampling tube. Not every sample collection place can be detected by PCR. Therefore, the collected virus swab samples need to be transported. After the samples are sent to the detection institution, they are analyzed by PCR amplification and other methods, which belongs to the nucleic acid detection of virus.

VTM Virus Transport Media for nucleic acid detection

The sampling tube for virus sample collection is composed of sampling tube, sampling swab (nose and throat swab) and Virus Transport Media added in the tube. The preservation solution is divided into inactivated type and non inactivated type:

1. Inactivated preservation solution: it is composed of high concentration of cleavage salt, which can not only quickly destroy the cell membrane of the virus host cell, but also denature the protein to denature and precipitate the virus protein, so that the nucleic acid can get rid of the protein entanglement and release the nucleic acid; It also contains buffer EDTA and Tris and nuclease inhibitors.

2. Non inactivated preservation solution: it consists of Hanks solution, which is mainly made of a variety of inorganic salts, buffers, glucose and 0.4% phenol red under sterilization conditions, which is consistent with the pH value, osmotic pressure and sterile conditions of cell growth state, so as to prolong the survival time of virus host cells in vitro; In addition, it also contains protein protectants to protect virus stability, buffers and cryoprotectors.

Use of Virus Transport Media and sampling tube:

a) Nasal collection: gently insert the swab head into the nasal cavity for sampling, use another swab to collect another nasal sample in the same way, immerse the swab head in the sampling solution and break the tail;

b) Throat collection: collect throat samples with a swab. Similarly, invade the swab head into the sampling solution and break the tail.

Hubei xindesheng Material Technology Co., Ltd. is located in Ezhou, Hubei Province, adjacent to Wuhan. At the most difficult time, Desheng developed virus preservation solution and widely used it to detect covid-19, which has been widely recognized by customers. We seem small in the face of the epidemic, but the power of mankind is infinite. Desheng hopes to give full play to its energy in this battle, And help more people out of this haze.

What is the role of virus preservation solution in nucleic acid detection?
The epidemic in Shijiazhuang, Hebei has once again seen the coming of the epidemic. For everyone's safety, nucleic acid testing for all employees has been opened again. Speaking of nucleic acid testing, we have to mention our virus preservation solution. What is the virus preservation solution? What does it do?

The virus preservation solution is a protective liquid medium added to the virus sampling tube to protect the sample after the nasopharyngeal swab is sampled. It is usually called the virus preservation solution in China. Normally, nucleic acid PCR cannot be directly performed at the sample collection site during nucleic acid detection. If the sample collected by the swab needs to be transferred and transported, it is necessary to add a virus preservation solution.
However, according to different detection conditions and requirements, the virus preservation solution is divided into two types: inactivated and non-inactivated.

Inactivated virus preservation solution:
1. It is mainly a virus lysis storage solution modified from nucleic acid extraction lysis solution, which is added with a high concentration of lysis salt, which can quickly and efficiently inactivate the virus protein of the sample to be tested;
2. It can effectively prevent the operator from second infection;
3. At the same time, it contains Rnase enzyme inhibitor, which can protect viral nucleic acid from being degraded;
4. Moreover, it can be stored for a relatively long time at room temperature, which saves the cost of virus sample storage and transportation.
Non-inactivated preservation solution:
1. It is mainly a virus maintenance liquid type preservation solution that is improved based on the delivery medium;
2. This one retains the protein coat of the virus and the viral nucleic acid DNA or RNA at the same time;
3. In this way, the virus has protein antigen epitope and nucleic acid integrity in vitro;
4. Of course, there is a certain infectious risk when operating errors. However, it maintains the originality of the virus sample to the greatest extent, and it needs to be kept strictly low temperature for long-term storage after sampling.
Both types of preservation solutions are necessary, which are determined by different experimental purposes and different detection conditions. No matter which virus preservation solution is, it has its advantages. When we choose, we must choose according to the actual situation. You may feel that the non-inactivated preservation solution has certain risks, but it is safe as long as protective measures are taken.