How To Use EDTA Tripotassium In Vacuum Blood Collection Tubes

How to use EDTA tripotassium in vacuum blood collection tubes

EDTA is also called ethylenediaminetetraacetic acid. As an amino polyacid, it often exists in the form of potassium salt, such as: EDTA dipotassium and EDTA tripotassium. Because it can chelate with most metal ions and has stable performance, it is often prepared as a solvent and added to vacuum blood collection tubes to prevent blood coagulation.

1. The use of tripotassium EDTA

Tripotassium EDTA is used as an in vitro blood anticoagulant. It is often added to purple anticoagulant tubes and used with blood analysis instruments. It is especially suitable for various clinical emergency blood tests. Because tripotassium EDTA has no effect on blood cells and can prevent platelet aggregation and adhesion, it is considered an ideal anticoagulant. However, it should be noted that due to the anticoagulant function, it is not suitable for use in coagulation tests and platelet tests. After use, the test results are not obvious or there are errors.

EDTA tripotassium parameters:

2. the addition of EDTA tripotassium

Since the produced tripotassium EDTA is in the form of white crystalline powder, it needs to be configured as a solvent when it is added to vacuum blood collection. Usually, deionized water is used for dissolving, and the solubility of tripotassium EDTA must be as high as 60%. After dissolving, there are two ways to add it, namely machine and manual.

Machine addition requires all equipment to be cleaned. If necessary, use 80% alcohol to clean the container and pipe three times, and dry it with hot air to ensure that no impurities and water adhere to the pipe wall to avoid dilution caused by tripotassium. Manual addition should also ensure that the container and tools are cleaned. At this time, it is necessary to pay attention to the requirements of adding solution during manual operation to avoid too much or too little solution.

3. Precautions for the use of tripotassium EDTA

Tripotassium EDTA is added to the purple anticoagulant tube. Although the anticoagulation effect is good, some problems need to be paid attention to. For example, if the blood collection environment is relatively high, above 25 degrees, it is necessary to pay attention to the blood must be mixed with tripotassium EDTA. Make the blood fully exposed to the solvent, otherwise half of it will coagulate and half will not coagulate. In addition, anticoagulant tubes using tripotassium EDTA should not be stored for more than 24 hours after blood collection, and the test should be completed as soon as possible.

Desheng Biochemical is committed to the R & D and production of blood collection tube reagents. As an advantageous supplier, its EDTA dipotassium and tripotassium have a purity of over 99% and stable performance. It has cooperated with more than 100 companies at home and abroad, and its quality is widely recognized. If you have a demand for blood collection tube reagents, please visit our website !