Blood Anticoagulant EDTA Dipotassium Dihydrate CAS25102-12-9

Blood Anticoagulant EDTA Dipotassium Dihydrate CAS25102-12-9

The dissolving method step of EDTA k2

Nowadays, when major hospitals check patients for diseases, they will conduct blood tests, and judge whether they are sick or not according to the blood cell components. As a routine blood test, in order to improve the detection efficiency, other substances are needed to assist at this time. Among them, dipotassium edetate is a common anticoagulant substance in blood analysis. It is widely used in clinical medicine because of its good anticoagulant effect and little effect on blood cell morphology.

Dipotassium EDTA parameters:

Dipotassium ethylenediaminetetraacetic acid is also known as EDTA-K2, which belongs to a kind of EDTA salt. It is mainly a kind of crystal obtained by the reaction of EDTA and potassium hydroxide, and then dehydrated and dried to develop into a powdery finished product. When used, the powder needs to be formulated into a solution, and then sprayed on the inner wall of the test tube to have an anticoagulant effect. So, how to dissolve it from powder to solution? The following is a detailed introduction to the dissolving method steps of dipotassium EDTA, and you can refer to complete the configuration.

1. Determine the dosage

The most critical step is to determine the amount of blood collected in the test tube in order to obtain the amount of EDTA-K2. According to the research data, the content of EDTA-K2 in each milliliter of blood is 1.5-2.2 mg, so before making the configuration, it is necessary to calculate the dosage to avoid waste of raw materials or abnormal detection results.

2. Configuration steps

Step 1: Weigh the required amount of EDTA-K2 powder on the electronic balance scale;

Step 2: Pour a part of the weighed powder into distilled water or deionized water for dissolution. During the dissolution process, it needs to be stirred continuously, and then slowly pour in the remaining EDTA-K2 powder while stirring;

Step 3: After the powder is completely dissolved, add deionized water or distilled water again, and adjust the volume to the required amount.

Step 4: Put the fixed volume solution into a sealed container, and slowly invert and mix six or seven times.

However, it should be noted that when making a solution, the required concentration must be confirmed clearly, because too high a concentration may increase the local osmotic pressure, cause local blood cell rupture and cause hemolysis, and affect the use process. In addition, the prepared solution can be stored for up to one week at minus 4 degrees Celsius, so it is recommended to use it immediately.

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