Different Types Of Blood Sampling Additives In Vacuum Blood Collection

The additives used in vacuum test tubes are important auxiliary reagents in blood testing. Their types, performance differences, and concentrations directly affect the detection effect of blood samples. Among them, the more common additives are coagulants, anticoagulants, Serum separating gel, and silicifying agents. So let's focus on introducing them to everyone, and we can also use them specifically in the detection process.

Serum separating gel

1, Serum separating gel

Serum separation gel is added as an additive to a test tube, and it can utilize its own performance to achieve separation effects. When it is centrifuged in the same test tube as blood, it quickly turns over to the center of the test tube, separating the serum and blood clots, preventing them from interacting, and ensuring the stability of serum components. At the same time, it can ensure that there is no change in the blood during transportation or storage, which can promote more accurate blood testing.

2, Heparin sodium/heparin lithium

As a natural and excellent anticoagulant, heparin can be made into heparin sodium or heparin lithium for use. Because it does not affect blood components, does not interfere with red blood cell volume, and does not cause hemolysis, it is suitable for blood gas analysis, erythrocyte sedimentation rate determination, and other tests. Adding them to a test tube is conducive to preventing blood clotting, achieving the goal of rapid separation of plasma, and the resulting samples are suitable for long-term storage.

Accelerator parameters:

3, EDTA potassium salt

Potassium EDTA salts contain two substances, potassium EDTA and potassium EDTA, both of which belong to the type of anticoagulants. Because they both contain substances such as EDTA, they have an affinity for calcium in the blood and can be complexed to cause non coagulation in the blood. Typically, an EDTA anticoagulant of 1.0-2.0 mg can prevent 1 ml of blood from clotting. This anticoagulant has little impact on the size of white blood cell counts, and has minimal impact on the morphology of red blood cells. It can fully inhibit platelet aggregation. It is suitable for routine blood testing and is also recognized as an ideal anticoagulant in medicine.

4, Coagulant accelerator

Adding a coagulant promoter to a test tube is mainly to accelerate blood coagulation without affecting the composition of the blood sample and further promote serum separation. Its coagulation promoting mechanism is also relatively simple, mainly by activating plasma and coagulation factors to accelerate blood cell clotting, thereby achieving the goal of promoting coagulation. Generally, when adding the reagent, the spray method will be used to evenly spray the reagent on the inner wall of the test tube, and then gently rotate it to the four sides, which is conducive to the rapid mixing of blood samples.

5, Silicifying agent

In addition, a silicifying agent is also added to the test tube, which is generally used in conjunction with other additives to ensure that the inner wall of the test tube is smooth, transparent, and not rough, and that there is no wall hanging phenomenon at the nozzle when collecting blood samples. Spray and dip coating methods are often used to uniformly adhere the reagent to the tube wall, facilitating the full mixing of blood samples after sampling. Due to its smoothness and non adherence to the wall, the detection efficiency is improved to a certain extent, and the protection of blood cells from external damage is ensured.

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