Luminol CAS 521-31-3 Chemiluminescence Reagents

Luminol CAS 521-31-3 Chemiluminescence Immunoassay reagent
 

Luminol；3-Aminophthalhydrazide；Chemiluminescent Reagent；521-31-3；off-white powder；Chemiluminescence immunoassay

What is luminol and its luminescence principle

Keywords: Luminol, Chemiluminescence
Luminol sodium salt is a white to light yellow crystalline powder prepared by reacting luminol with sodium hydroxide. Luminol is one of the most commonly used liquid phase chemiluminescence reagents. Since Albrecht first reported the chemiluminescence reaction of luminol and oxidant in alkaline solution in 1928, the research on the chemiluminescence system has been very active, making the chemiluminescence system be used in many fields. Luminol sodium is one of the application extensions of luminol's luminescence properties.

Basic information

Lumino
What is luminol
Luminol is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst. This catalyst is generally For multivalent metal ions, peroxidases such as iron, horseradish peroxidase, etc., this method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals Toxic analysis and analysis methods based on peroxidase and glucose oxidase.

Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity. The second is to use the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol to determine the organic compounds that have a quenching effect on the chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reactions.

At present, the main catalysts of luminol include some metal complexes and transition metal ions. The metal complexes mainly include hemoglobin and peroxidase; the transition metal ions mainly include Fe3+, Fe2+, Mn2+, Cr2+, etc. The catalyst that we often use in chemiluminescence immunoassay is peroxide, especially horseradish peroxidase. Some compounds can be immunoreacted with peroxidase-labeled antibodies, and then chemiluminescence assays can be performed with luminol to determine these compounds or antibodies. Such as digoxin, hepatitis B surface antigen, etc. are all detected and analyzed by this method.

In addition to the catalyst’s acceleration of detection, some inhibitors were also found in the research. These organic compounds can inhibit the chemiluminescence of luminol, such as reducing compounds containing phenolic hydroxyl groups, which can react with oxidants during the reaction, reducing the oxidants. The concentration of, the intensity of chemiluminescence is reduced, so as to indirectly measure this kind of organic substances.

Another method is to perform indirect determination through a coupling reaction. The coupling reaction here refers to combining a reaction that can produce or consume a chemiluminescent reactant with another chemiluminescent reaction, so as to achieve indirect chemiluminescence of certain substances. Determination. For example, some substrates produce hydrogen peroxide under the action of certain enzymes and then produce chemiluminescence with luminol. By measuring chemiluminescence, the purity of the measured substrate can be indirectly known.

Hubei New Desheng Materials specializes in the production and development of chemiluminescence reagents, luminescent substrates, biological buffers, blood collection tube additives and enzyme preparations. Desheng has been established for decades and has its own R&D team. It has been researching and developing chemiluminescence reagents for many years. It has many kinds of raw chemical products under its umbrella. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.