EDTA K3 Tripotassium Salts Blood Collection Tubes Additives

EDTA K3 Tripotassium Salts blood collection tubes additives

[product name] tri potassium EDTA dihydrate/EDTA K3

[introduction] What is K3 EDTA?

Potassium salts of EDTA are used as anticougulating agents for the preservation of whole blood. This can then be used for routine haematology tests including Complete Blood Count (CBC), Haemoglobin and Haematocrit, WBC count, RBC count, Platelet count, and HbA1C test. The special formulation also prevents platelet aggregation of the whole blood.

The tubes contain EDTA, preventing blood from clotting by binding calcium ions.

Anticoagulant: EDTA potassium salt (ethylenediaminetetraacetate).

Specimen: whole blood.
Immediately after the specimen is collected, gently invert the EDTA tube 9-10 times to mix thoroughly.
Insufficient mixing could lead to platelet aggregation, microclot formation or coagulation.

[Formula] edtak3 · 2H2O

[package specification] plastic bottle, 500g / bottle

[product performance] white crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3 ± 1 (5% aqueous solution, g / ml,), solubility in water ≥ 60% (g / ml, 25 ℃). Stable at room temperature. The main content of EDTA in the company is ≥ 99.0%. See the product test report for other indicators.

[background] the EDTA K3 Blood Collection Tube is available with a pink top, a lavender top or white top blood tube and is used primarily in hematology and blood banking. The inner wall of blood bank tube is coated with spray-dried K3 EDTA additive which acts as an anticoagulant, binding calcium ions and interrupting the clotting cascade.

[main use] this product is specially used for anticoagulation of blood in vitro. It is an anticoagulant additive for clinical blood test. It is used for blood sample pretreatment (anticoagulation treatment) in the process of clinical blood collection and test.

[scope of application] EDTA salt can protect the cell composition of blood, not affect the white blood cell count and size, has the least impact on the morphology of red blood cells, and can inhibit the aggregation of platelets, which is suitable for general hematology test. In addition to the separation and test of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test.

The International Committee for standardization of Hematology (ICSH) recommended EDTA as the anticoagulant for blood cell count and classification. Compared with EDTA, it has higher solubility and faster anticoagulation speed.

[warning and prevention]

When impurities, foreign matters, abnormal smell, color, serious moisture absorption and expiration date occur in EDTA, it is forbidden to use.

Do not continue to use EDTA Tripotassium solution in case of turbidity.

This product is a chemical reagent with strong corrosiveness. It is forbidden to touch it directly. When splashing on the skin, wash with plenty of water. Splashing into eyes can be washed with plenty of water immediately. In case of emergency, it should be sent to hospital for examination and treatment immediately.

[storage conditions] the product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed and stored in sterile state at room temperature. It is recommended that the aqueous solution of this product be prepared and used immediately, and not stored in solution state for a long time.

[term of validity] 3 years

Product name	Tri-potassium EDTA
Formula	C10H17K3N2O10	HS Code	2921219000
Molecular weight	442.54	Cas no	65501-24-8
Structure		EINECS no	241-543-5
		Melting point	182ºC
		Boiling point	182ºC(DEC.)
Appearance	Powder	Color	White
Odor	odorless	Solubility	≥60%
Content	≥99.0%	Chloride	≤0.005%
Sulfate	≤0.02%	Iron	≤0.001%
Copper	≤0.0025%	Heavy metal	≤0.001%
Danger classification	R36/37/38	Safety classification	S26, S37/39
Packing way	O53	Packing size	500g/bottle
Application	Blood collection tube additive

[Vacuum EDTA tubes FAQ]

Q:What does EDTA stand for and how does the anticoagulant work?

A:EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium in the blood and keeping the blood from clotting.

Q:What is the concentration of EDTA in the lavender and pink stoppered tubes?

A:The nominal EDTA concentration is 1.8mg EDTA per milliliter of blood.

Q:Why do some Vacuum lavender top blood collection tubes contain K2EDTA and others contain K3EDTA?

A:Blood Collection Tubes contain K2EDTA, which is spray-dried to the walls of the tube. K2EDTA is recommended by the CLSI (CLSI guideline - Evacuated Tubes and Additives for Blood Specimen Collection-Fifth Edition H1-A5, 2003) and the ICSH (International Council for Standardization in Hematology). Vacuum glass blood collection tubes contain K3EDTA, the liquid form of EDTA.

Q:What differences can I expect to see in hematological parameters when I switch from Vacuum K3EDTA to Vacuum K2EDTA?

A:K3EDTA is a liquid and will dilute the sample ~ 1-2%. K2EDTA is spray-dried on the walls of the tube and will not dilute the sample. Slightly higher results have been seen from the K2EDTA tube in some CBC parameters, but none of these were clinically significant. It is important to mix the sample 8 - 10 times immediately after collection to ensure all the anticoagulant is thoroughly mixed with the blood sample.

Q:Can the Vacuum Plus Plastic EDTA Tube be used for routine blood bank procedures?

A:Yes, the Vacuum K2EDTA Plus Tubes are intended for routine immunohematology testing, including red cell grouping, Rh typing and antibody screening.

Q:What are the differences between the lavender stopper and the pink stopper BD Vacuum K2EDTA Tubes?

A:The differences are the types of closures and the labeling. The lavender stopper can either be rubber or a Hemogard™ closure on a plastic tube. Product 367899, a 6 mL plastic tube, and product 367842, a 2 mL plastic tube, both have a distinct pink BD Hemogard™ closure and product 368589 has a conventional rubber pink stopper. The pink stoppered tubes have blood bank labels and are generally sent to the blood bank laboratory in the hospital. The additive is the same in both tubes.

Q:What is the minimum and maximum fill volume in the EDTA tubes?

A:The minimum and maximum acceptable blood volumes should be established by each facility, to ensure that accurate hematology results are obtained. BD Vacuum blood collection tubes are designed to draw the appropriate volume to ensure a proper blood to additive ratio.