Green Top Blood Tube Heparin Sodium Bioassay Additives For Blood Collection

Heparin sodium is an important reagent used in clinical medicine and biological research, so the bioassay of heparin sodium is crucial for evaluating its activity, efficacy, and quality control. In addition, due to the fact that the activity of heparin is generally achieved through its interaction with antithrombin or coagulation factors, direct functional evaluation can be provided using bioassay methods, which can more accurately reflect the anticoagulant performance of heparin sodium compared to other physical and chemical methods. Below, we will focus on introducing this aspect in detail.

Heparin sodium parameters:

1, Clotting time method

This is a common bioassay method for heparin sodium. It is based on the principle of heparin sodium inhibiting thrombin activity, adding thrombin and clotting substrates (such as prothrombin) to plasma or serum samples, and adding different concentrations of heparin sodium solutions to determine the clotting time in the sample, which is the time required for plasma or serum to begin clotting. By comparing with standard heparin sodium samples, the anticoagulant activity of heparin in unknown samples can be determined.

Heparin sodium powder

2, Anticoagulant activity determination method

This method evaluates the anticoagulant activity of heparin sodium by measuring its inhibitory effect on thrombin activity. It usually uses indicators such as enzymatic clotting time (ETT) or activated partial thromboplastin time (APTT) to measure the degree of prolongation of clotting time by placing plasma or serum samples in the same environment as heparin sodium. By comparing with standard heparin sodium samples, the anticoagulant activity of heparin in unknown samples can be determined.

3, Sheep plasma assay method

The sheep plasma method mainly measures its potency. Generally, the potency of heparin sodium added to the blood collection vessel ranges from 150IU-180IU. By measuring the potency, it can be determined whether it meets the experimental requirements. By adding sodium citrate solution and calcium chloride solution to different specifications of test tubes, filtered sheep plasma is placed in a 37 degree water bath, and the semi freezing point is recorded to draw a conclusion.

As a supplier of raw materials for blood vessel additives, Desheng has established a research and development department and a quality department specifically for product quality. Factory goods must undergo repeated sampling before they can be sold. Whether measured or used, customers are very satisfied with their performance. If you are also interested, please click on the website for consultation!