Biological Buffer 4-Hydroxyethyl Piperazine Ethanesulfonic Acid 7635-45-9

4-Hydroxyethylpiperazine Ethanesulfonic acid, named HEPES in English, CAS No. 7635-45-9, is a white crystalline powder. It is a commonly used buffer, not only in biochemical and molecular biology experiments, but also in cosmetics.

Parameters of 4-hydroxyethyl piperazine Ethanesulfonic acid:

HEPES Chemical Properties

HEPES is a white crystalline powder with the chemical formula C8H18N2O4S and a relative molecular weight of 238.3 g/mol. It contains a piperazine ring, a hydroxyethyl group, and a sulfonic acid group. It has good solubility in water, with a pKa value of approximately 7.55, making it suitable for buffer solutions in the neutral pH range.

Characteristics of HEPES

1. Neutral pH range: The pKa value of HEPES is close to the physiological pH value, making it suitable for many experiments that require neutral conditions in biological research.

2. Low adhesion: It has low adhesion and can reduce interference with experimental samples, especially suitable for experiments that require maintaining sample integrity and stability.

3. Temperature stability: It can maintain relative stability at conventional laboratory temperatures and is not prone to thermal decomposition, making it an ideal choice for long-term experiments and storage periods.

4. Biocompatibility: HEPES buffer has good biocompatibility with many biological systems and does not cause significant toxicity or interference to cells and biomolecules.

HEPES Powder

HEPES application

1. Buffer: HEPES, as an effective buffer, can maintain solution stability and appropriate pH values in biochemical and molecular biology experiments. It is commonly used in fields such as cell culture, enzyme activity analysis, and protein research.

2. Cell culture: HEPES can stabilize the pH value of cell culture medium, provide suitable environmental conditions, and maintain cell growth and function.

3. DNA/RNA extraction: it can be used as a buffer in the process of DNA/RNA extraction, helping to maintain proper acid-base balance and improve the extraction effect.

4. Enzyme activity research: Due to its pH range and good buffering performance, it is also commonly used in enzyme activity research to ensure that the enzyme has the best activity under suitable conditions.

4-hydroxyethyl piperazine Ethanesulfonic acid is an important buffer, which has a wide range of applications and advantages. It is an ideal choice for experiments. As a professional manufacturer of biological buffer, Desheng's products are analytical grade and are not only suitable for various experimental fields, but also have obtained submission codes, which can be used in cosmetics with stable performance. If you are interested, please feel free to contact us for purchase!