Cas 7365-45-9 HEPES Buffer Solution 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid

Cas 7365-45-9 HEPES 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid

What is HEPES

HEPES as one of more than 20 kinds of good's reagents, HEPES is more used in cell culture, more broadly classified into biological buffers. The full name is 4-hydroxyethyl piperazine ethanesulfonic acid, also known as 2 - [4 - (hydroxyethyl) - 1-piperazinyl] ethanesulfonic acid, which is a zwitterionic sulfonic acid buffer. In 1966, Norman E. good proposed that HEPES is a good's buffer.

Preparation of different types of buffers for hydroxyethylpiperazine ethanesulfonic acid HEPES

HEPES, 4-hydroxyethylpiperazine ethanesulfonic acid, is a commonly used biological buffer, usually used in cell preservation solutions, DNA/RNA extraction kits, and PCR diagnostic kits. According to different needs, HEPES can be configured into a variety of different buffer systems.

HEPES buffer has many advantages, good buffering performance, and non-toxic to cells. It is a hydrogen ion buffer that can control the system in a constant pH range for a long time. Its pH buffer range is 6.8-8.2, and the final concentration is 10-50mmol/L. Generally, the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.

Buffer range of HEPES

Most buffers realize their own "value" through their own buffer range. The pH range of buffer capacity that can be exerted by the buffer as a solvent is the buffer range. The buffer range of HEPES is 6.8 ~ 8.2, and the pKa is 7.48 at 25 ℃. The buffer ratio is 0.1-1.0. If it exceeds the buffer range, it is generally regarded as losing the function of buffer.

The use of HEPES

HEPES is more commonly used in cell culture, in vitro fertilization and embryo culture buffer, but HEPES can be combined with metal ions, most of the metal ion research is carried out by using HEPES buffer, although Tris and phosphate can also be used for metal ion research, but based on the characteristics of these buffers, it is necessary to choose different directions There will be some differences.

Nowadays, more and more attention is paid to the protection of the environment all over the world, and the analysis and some biological research in the environment is the use of HEPES buffer, more industrial grade HEPES. As a binding buffer and eluent in cation exchange, it is also used for grinding in plant research and development. In addition, HEPES is electrophoretic buffer, which is also commonly used in electroporation.

HEPES has a lot of functions, which will not be repeated here. The development of HEPES will be further expanded in the future.

Biological buffer HEPES
Due to the better performance of HEPES in cell culture media, research has found that it is also suitable for use as a buffer in human peripheral blood lymphocyte cultures, and has the following advantages:
1. It is non-toxic to lymphocytes in the concentration range (10-40 m M) and has excellent buffering capacity.
2. It does not stimulate lymphocytes by itself.
3. It basically does not affect the lymphocyte stimulation rate induced by non-specific stimulants (phytohemagglutinin), antigens (tuberculin) or mixed allogeneic cultures.

Compared with another bicarbonate buffer commonly used in cell culture, it provides greater buffering capacity in the physiological range of pH. Therefore, even in very crowded cultures, HEPES buffered medium can maintain a constant pH value, ensuring better cell survival and stimulation. PHA-induced lymphocytes were observed to produce great stimulation in HEPES buffered cultures in the pH range of 7.4-7.6.

The above briefly describes the advantages of HEPES in the field of cell culture, which is why HEPES is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological and biochemical research. Desheng Company is a company specializing in the production of biological buffers. It has been engaged in the research and development and production of HEPES for more than ten years. The product knowledge is very rich. Please feel free to contact us if you need it!