EDTA K3 EDTA Salts for blood collection tubes blood testing

EDTA K3 EDTA Salts for blood collection tubes blood testing

Tripotassium ethylenediaminetetraacetic acid is a kind of potassium salt of ethylenediaminetetraacetic acid EDTA. It can form superfluous compound with most metal ions and become a general-purpose strong superfluous agent, used for extraction, cation exchange, rare elements Separation, volumetric analysis of calcium, magnesium, and barium, hard water softening, and use as a masking agent, metal cleaning agent, antidote for heavy metal poisoning, and a bactericide.

Physical and chemical properties

The structure of tripotassium ethylenediaminetetraacetate (EDTA-Tripotassium)

High-purity tripotassium ethylenediaminetetraacetate is usually used as an anticoagulant in vacuum blood collection tubes for blood cell analysis. The use of ethylenediaminetetraacetic acid (EDTA) salt as an anticoagulant for blood cell analysis was determined by the International Committee for Blood Standards (ICSH) in 1993 and is widely used.

Using EDTA-K3 anticoagulant blood for blood analysis, there are few reports about EDTA-dependent platelet false reduction. Studies have shown that unexplained low platelet counts are found when using EDTA-K3 anticoagulant blood for blood analysis As a result, the patient should be manually inspected blood slices and PLT manual count review, or the PLT count should be reviewed directly with the instrument for blood cell analysis after dilution with unanticoagulated peripheral blood, in order to correctly understand the patient's true level of PLT. Especially patients with tumors, autoimmune diseases, pulmonary heart disease, pregnant women, advanced liver disease, etc.

In addition to being used as an anticoagulant, tripotassium ethylenediaminetetraacetate is also similar to dipotassium ethylenediaminetetraacetate and disodium EDTA. It can be used with biological buffers such as Tris and MOPS. It can be used as a mask for some divalent metal ions. Agent, complexing agent, etc.

[introduction] What is K3 EDTA?

Potassium salts of EDTA are used as anticougulating agents for the preservation of whole blood. This can then be used for routine haematology tests including Complete Blood Count (CBC), Haemoglobin and Haematocrit, WBC count, RBC count, Platelet count, and HbA1C test. The special formulation also prevents platelet aggregation of the whole blood.

The tubes contain EDTA, preventing blood from clotting by binding calcium ions.

Anticoagulant: EDTA potassium salt (ethylenediaminetetraacetate).

Specimen: whole blood.
Immediately after the specimen is collected, gently invert the EDTA tube 9-10 times to mix thoroughly.
Insufficient mixing could lead to platelet aggregation, microclot formation or coagulation.

[Formula] edtak3 · 2H2O

[package specification] plastic bottle, 500g / bottle

[product performance] white crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3 ± 1 (5% aqueous solution, g / ml,), solubility in water ≥ 60% (g / ml, 25 ℃). Stable at room temperature. The main content of EDTA in the company is ≥ 99.0%. See the product test report for other indicators.

Product name	Tri-potassium EDTA
Formula	C10H17K3N2O10	HS Code	2921219000
Molecular weight	442.54	Cas no	65501-24-8
Structure		EINECS no	241-543-5
		Melting point	182ºC
		Boiling point	182ºC(DEC.)
Appearance	Powder	Color	White
Odor	odorless	Solubility	≥60%
Content	≥99.0%	Chloride	≤0.005%
Sulfate	≤0.02%	Iron	≤0.001%
Copper	≤0.0025%	Heavy metal	≤0.001%
Danger classification	R36/37/38	Safety classification	S26, S37/39
Packing way	O53	Packing size	500g/bottle
Application	Blood collection tube additive

P.S.[What is the Difference Between K2 EDTA and K3 EDTA?]

The main difference between K2 EDTA and K3 EDTA is that K2 EDTA contains two chelated potassium ions whereas K3 EDTA contains three chelated potassium ions. Furthermore, K2 EDTA slightly increases the MCV (mean corpuscular volume) of red blood cells at higher concentrations while K3 EDTA has no influence on the MCV of red blood cells at higher concentrations.

K2 EDTA and K3 EDTA are two types of anticoagulants used in routine hematological tests. However, their influence on the blood count remains controversial.