Analytical Pure Level High Purity White Powder Hepes Buffer In Cell Culture Medium

Cell culture is an indispensable part of modern life science research, and HEPES buffer, as a widely used buffer, has been applied in cell culture media. It can maintain the stability and appropriate pH value of the culture medium, provide the basic environment required for cell growth and reproduction, and play a crucial role in stabilizing cell culture. Below is a detailed introduction to the role and advantages of HEPES buffer in cell culture medium.

HEPES buffer parameters:

1, Maintain a stable pH value

PH value is a key factor in cell growth and metabolism, and high or low pH values can affect the growth function of cells. And HEPES buffer has good buffering ability, with a pH range of 6.8-8.2, which can effectively maintain the pH value of the culture medium within an appropriate range and provide a stable growth environment.

2, Avoiding the problem of carbon dioxide (CO2) dissolution

In cell culture, cell metabolism produces carbon dioxide, leading to a decrease in the pH of the culture medium. Adding HEPES buffer can help stabilize pH, reduce pH changes caused by carbon dioxide, and maintain carbon dioxide balance in the culture medium.

3, Less impact on cells

HEPES buffer solution does not have harmful effects on cells in cell culture medium, and can interact stably with cells to maintain normal growth and function.

4, Suitable for multiple cell types

HEPES buffer is suitable for the cultivation of various cell types, including mammalian cells, insect cells, cell lysis, live cell imaging, etc., making it an excellent choice widely applicable for various cell research.

5, Stable environment

Unlike some other buffer solutions, HEPES buffer solution does not need to be used in environments containing high concentrations of carbon dioxide, which has advantages under special cultivation conditions. At the same time, it has high stability in cell culture medium and is not easily affected by external factors.

Hepes Powder

HEPES buffer has the advantages of maintaining pH stability and minimal impact on cells in cell culture. It provides a stable growth environment for cell research, which helps to ensure the accuracy and repeatability of experiments. When selecting cell culture medium buffer, researchers can consider adding HEPES buffer to meet the needs of cell culture.

As a supplier of HEPES, Desheng can provide biological buffer raw materials with a purity of up to 99%, convenient transportation, simple preparation, and is conducive to various biochemical experiments. Its performance is stable and is the choice of many people. If you are also interested, please feel free to contact us at any time!