77-86-1 Trihydroxymethyl Aminomethane Buffer For Biological Buffers

Tris buffer solution, also known as trimethylaminomethane, is a commonly used buffer solution in biochemical experiments. It has the characteristics of stable acid-base environment and is commonly used in experiments in fields such as molecular biology, biochemistry, and cell biology. Its composition affects its acid-base buffering performance, which has a significant impact on the experimental results.

Trihydroxymethyl aminomethane parameters:

Trihydroxymethyl aminomethane(tris)

Trihydroxymethylaminomethane and hydrochloric acid are the core components of tris buffer and the source of their names. It is a weakly alkaline amino alcohol with the molecular formula C4H11NO3. Its chemical structure contains three hydroxyl groups and one amino group, which endows it with good acid-base buffering performance. In tris buffer, trimethylaminomethane maintains the pH stability of the solution by accepting or releasing hydrogen ions.

Preparation of Tris buffer

When preparing tris buffer, it is often necessary to use acids or bases to adjust its pH value to the desired range. For example, if a buffer with a pH of 7.4 needs to be prepared, hydrochloric acid or sodium hydroxide can be used to adjust it. Deionized water is the solvent for tris buffer, ensuring the full dissolution and stability of other components. The preparation steps are as follows:

(1) Firstly, prepare the required solid powder of Tris.

(2) Add an appropriate amount of trihydroxymethylaminomethane powder to an appropriate amount of deionized water, stir and dissolve.

(3) Add acid or alkali, such as hydrochloric acid or sodium hydroxide, dropwise to the solution while monitoring the pH value with a pH meter until the desired pH range is reached.

(4) Finally, dilute the solution volume with deionized water to the desired concentration.

tris powder

Preparation precautions

It should be noted that during the preparation process, the operating environment should be kept clean to avoid interference from external pollution on the experimental results. In addition, to ensure the accuracy of experimental results, it is best to use standard working solutions and standard curves to verify the buffering capacity and pH value of the prepared tris buffer.

In summary, the composition of tris buffer mainly includes trimethylaminomethane and hydrochloric acid. If the proportion of these components is reasonably controlled, tris buffer with good stability and buffering ability can be prepared for various biochemical experiments.

As a professional manufacturer of Tris, Desheng can provide white crystal powder raw materials with a purity of up to 99% and a pure appearance. When used, it only needs to be prepared, which is convenient and efficient, effectively saving time, and improving production efficiency. If you have any interest, please feel free to contact us for purchase!