Blood Collection Tube Additive EDTA-K3 Purple Hat 65501-24-8

Ethylenediaminetetraacetic acid tripotassium dihydrate is a white crystalline powder that is easily soluble in water and insoluble in organic solvents such as ethanol. Its molecular formula is C10H12N2O8P2 · 3K · 2H2O, with a molecular weight of 446.37. Ethylenediaminetetraacetic acid tripotassium dihydrate is stable in a dry state, but is prone to deliquescence in humid environments. This article will provide a detailed introduction to this substance.

Application area

1. Anticoagulant in blood collection: Ethylenediaminetetraacetic acid tripotassium dihydrate is a commonly used anticoagulant in blood collection, which chelates calcium ions in blood samples to maintain their original appearance for a certain period of time.

2. Protein purification: During the protein purification process, ethylenediaminetetraacetic acid tripotassium dihydrate can chelate multiple metal ions to form stable complexes, thereby eliminating the interference of metal ions on proteins and improving protein purity and stability.

3. DNA/RNA extraction: Ethylenediaminetetraacetic acid tripotassium dihydrate can effectively chelate metal ions in DNA/RNA solution, providing stable pH and ion strength, thereby ensuring the stability and reliability of the buffer.

4. Preparation of buffer solution: Ethylenediaminetetraacetic acid tripotassium dihydrate can be used as one of the components for preparing buffer solution, providing stable pH value and ion strength, ensuring the stability and reliability of the buffer solution.

Ethylenediaminetetraacetic acid tripotassium dihydrate powder

Preparation method

There are two main methods for preparing ethylenediaminetetraacetic acid tripotassium dihydrate: chemical synthesis method and microbial fermentation method. The chemical synthesis method involves synthesizing raw materials such as ethylenediamine and p-chloroformic acid through multiple chemical reactions; The rule of microbial fermentation is to utilize certain microorganisms to produce under specific conditions. Both methods can obtain high-purity ethylenediaminetetraacetic acid tripotassium dihydrate, but microbial fermentation has the advantages of environmental protection and efficiency, gradually becoming the mainstream preparation method.

Quality standard

The quality standards for ethylenediaminetetraacetic acid tripotassium dihydrate mainly include appearance, purity, metal ion content, water content, and other indicators. For example, it should be ensured that the appearance is white or light yellow crystalline powder, the purity should comply with relevant standards such as HPLC, GC and other analytical methods, the metal ion content should be strictly controlled to ensure its stability in biological experiments, and the water content should also comply with relevant standards such as water titration, Karl Fischer method, etc. In addition, other indicators such as pH value and heavy metal content should also comply with relevant standards.

Matters needing attention

1. Storage: Potassium ethylenediaminetetraacetate dihydrate is more suitable for storage in a cool, dry, and ventilated environment, avoiding storage with strong acids, bases, and other substances. In addition, the humidity of the storage environment also needs to be controlled.

2. Usage: When using ethylenediaminetetraacetic acid tripotassium dihydrate, it is necessary to strictly follow the instructions for use, wear a mask, protective clothing, etc., and take protective measures.

Ethylenediaminetetraacetic acid tripotassium dihydrate, as a commonly used molecular biological reagent, plays an important role in laboratory and blood tests. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of research and development, production, and sales of blood collection additives. Currently, in addition to ethylenediaminetetraacetic acid tripotassium dihydrate, the products on sale include serum separation gel, heparin sodium, heparin lithium, coagulants, and silicides. If you are a manufacturer of blood collection tubes, please click on the Desheng official website to purchase the required blood collection tube additives!