Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 25kg / barrel
Delivery Time: 3 days
Payment Terms: L/C, T/T, D/P, Western Union
Supply Ability: 1t / day
CAS: |
64431-96-5 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
BIS-TRIS Propane |
CAS: |
64431-96-5 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
BIS-TRIS Propane |
In the field of life sciences, buffering agents are crucial reagents that play a crucial role in maintaining the stability of biological molecular structures and ensuring the smooth progress of biological reactions. Among them, BIS-TRIS propane, as a commonly used synthetic biological buffer, plays an indispensable role in many biological experiments. This article will delve into the material composition, synthesis methods, and applications of this buffer in the field of biological science.
Material composition of BIS-TRIS propane
BIS-TRIS propane is an organic compound with the molecular formula C10H22N2O12. This compound contains a large amount of hydroxyl and amino groups, which makes it highly water-soluble and buffering. Multiple hydroxyl and amino groups in its structure can form hydrogen bonds with water molecules, making it more water-soluble. In addition, due to its presence of multiple dissociative functional groups, it can effectively maintain pH stability.
| Chemical Name | 1,3-Bis[tris(hydroxymethyl)methylamino]propane |
| Abbreviation | BIS-TRIS propane |
| CAS NO | 64431-96-5 |
| Molecular formula | C11H26N2O6 |
| Molecular weight | 282.33 |
| Melting point | 164-165 °C(lit.) |
| Boiling point | 573.3±50.0 °C(Predicted) |
| Density | 1.326±0.06 g/cm3(Predicted) |
The synthesis method of BIS-TRIS propane
1. Preparation of starting materials: Corresponding starting materials need to be prepared, such as 1,3-propanediol, methyl chloroformate, etc. After appropriate treatment, these raw materials can be used for subsequent synthesis processes.
2. Esterification reaction: The starting material is esterified with an anhydride or carboxylic acid in the presence of an appropriate catalyst to generate corresponding ester intermediates. This step is a crucial step in the synthesis process, requiring strict control of reaction conditions to ensure the purity and yield of the product.
3. Ammoniation reaction: Reacting the ester intermediate generated in the previous step with ammonia or amine substances to introduce amino groups. This step also requires appropriate reaction conditions to ensure the quality of the product.
4. Hydrolysis and deprotection: Under the action of a catalyst, the product is subjected to hydrolysis and deprotection operations to obtain the target compound BIS-TRIS propane. This step requires precise control of hydrolysis and deprotection conditions to avoid product loss and byproduct generation.
5. Purification and crystallization: Separate the target compound from the reaction mixture through appropriate purification and crystallization operations. This step is an important step to ensure product purity and yield, usually requiring the use of appropriate solvents and crystallization conditions.
6. Throughout the entire synthesis process, it is necessary to strictly control the reaction conditions and operating parameters to ensure the purity and yield of the product. In addition, it is necessary to properly handle the waste and by-products generated during the reaction process to meet environmental requirements.
BIS-TRIS propane
The Application of BIS-TRIS Propane in the Field of Biological Sciences
1. Maintaining the stability of biomolecular structure: Due to its good buffering performance, BIS-TRIS propane can effectively maintain pH stability, making it suitable for maintaining the stability of biomolecular structure. For example, in protein crystal growth experiments, it can help stabilize the structure of proteins and promote crystal growth.
2. Ensuring the smooth progress of biological reactions: In some biological reaction systems, the stability of pH value is crucial for the smooth progress of the reaction. BIS-TRIS propane, as an efficient buffer, can provide a stable pH environment for these reactions, ensuring their smooth progress.
3. As solvents and media: In some biological experiments, suitable solvents and media are needed to dissolve or dilute biomolecules and reagents. BIS-TRIS propane has good water solubility and stability, and can be used as a solvent and medium in these experiments.
Hubei Xindesheng Material Technology Co., Ltd. has a history of nearly 20 years in the research and development of various biological buffering agents. It has a very mature technology for synthesizing BIS-TRIS propane, providing products with high purity, good water solubility, strong buffering ability, and ensuring affordable prices. If you have any related purchasing needs, please feel free to contact me!
