The conditions for luminescence of luminol monosodium salt 20666-12-0

Luminol monosodium salt is a type of chemiluminescent reagent. It is a strongly acidic cationic salt with hygroscopicity and is easily soluble in water and ethanol. In alkaline environments, luminol monosodium salt can be oxidized, producing chemiluminescence phenomenon. This characteristic makes it widely used in fields such as chemiluminescence analysis and fluorescence immunoassay.

The Luminescence Principle of Luminol Monosodium Salt

The luminescence principle of Luminol monosodium salt mainly involves energy conversion during chemical reaction processes. In alkaline environments, the monosodium salt of luminol is oxidized to form luminol. This process releases energy, putting Luminol in an excited state. When Luminol returns to the ground state, it will release energy in the form of light, producing blue fluorescence. This process is usually accompanied by the presence of oxygen in chemical reactions, which transfers electrons to the luminol monosodium salt through the reaction, promoting its luminescence.

Luminol monosodium salt powder

Luminescent Conditions of Luminol Monosodium Salt

1. Alkaline environment: Luminol monosodium salt needs to be oxidized in an alkaline environment, so substances such as sodium hydroxide and potassium hydroxide need to be added to provide the necessary alkaline conditions. The appropriate pH range is usually 11.0 to 12.3.

2. Temperature control: The luminescence process of luminol monosodium salt is affected by temperature. Excessive or insufficient temperature can lead to a decrease in luminous efficiency. Therefore, maintaining an appropriate temperature is an important factor in ensuring the luminescence effect of luminol monosodium salt. It is usually recommended to conduct experimental operations at room temperature to achieve good luminescence effects.

3. Concentration and purity: The concentration and purity of luminol monosodium salt can also affect its luminescence effect. Excessive concentration may lead to the formation of sediment, affecting the experimental results; If the concentration is too low, it may reduce the luminescence intensity. Therefore, during the experimental process, it is necessary to select a suitable concentration of luminol monosodium salt solution and ensure that its purity meets the experimental requirements.

Application fields of Luminol monosodium salt

1. Criminal investigation: In the field of criminal investigation, the luminescent properties of luminol monosodium salt can be used for blood testing and fingerprint detection. By spraying a solution of luminol monosodium salt on suspicious areas, trace blood traces can be detected, providing important clues for solving cases. In addition, the reaction of luminol monosodium salt with fingerprint residue can produce fluorescent fingerprints, which is helpful to determine the identity of suspect.

2. Environmental monitoring: In the field of environmental monitoring, luminol monosodium salt can be used to detect trace pollutants in water bodies. By adding a solution of luminol monosodium salt to the water sample and observing its luminescence, the content of harmful substances in the water can be quickly and conveniently detected, providing assistance for environmental protection work.

3. Bioanalysis: In the field of bioanalysis, luminol monosodium salt can be used to detect interactions between biomolecules and the concentration of biomolecules. By binding with specific biomolecules, luminol monosodium salt can generate fluorescence signals for monitoring the interactions between biomolecules and changes in biomolecular concentration.

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Hubei Xindesheng Material Technology Co., Ltd. is committed to the research and development of chemiluminescent reagents. We can provide high-purity luminol, isoluminol, luminol monosodium salt, and acridine ester series reagents. If you have any purchasing needs, please feel free to contact me!