Preparation and addition method of heparin lithium as an additive for blood collection

Lithium heparin, as a blood anticoagulant, mainly inhibits its activity by binding to thrombin in the blood, thereby preventing blood clotting. This allows blood samples to remain liquid after collection, facilitating subsequent laboratory processing and analysis. Meanwhile, heparin lithium also has a certain ion balance effect, which can maintain electrolyte balance in blood samples and ensure sample stability.

Preparation of heparin lithium raw material powder

Firstly, prepare the heparin lithium raw material powder. Ensure the quality of the powder is qualified, free of impurities, and has not expired. At the same time, prepare the necessary experimental equipment, including scales, volumetric flasks, beakers, glass rods, deionized water, etc., to ensure that all equipment is clean and pollution-free.

heparin lithium powder

Preparation of lithium heparin solution

1. Weighing: Use a balance to accurately weigh an appropriate amount of heparin lithium raw material powder. Determine the weight of the powder based on the required anticoagulant effect and the volume of the blood collection vessel.

2. Dissolution: Pour the weighed lithium heparin powder into a beaker and add an appropriate amount of deionized water. During the dissolution process, use a glass rod to continuously stir to ensure that the powder is evenly dispersed and dissolved.

3. Constant volume: After the powder is dissolved, transfer the solution to a volumetric flask. According to the specifications of the volumetric flask, use deionized water for constant volume until the liquid level reaches the mark.

4. Mix well: Gently shake the volumetric flask to mix the solution evenly. At this point, the lithium heparin solution has been prepared and can be added to the blood collection vessel.

Add lithium heparin solution to the blood collection vessel

1. Choose a blood collection vessel: Select the appropriate blood collection vessel according to the experimental requirements. Ensure that the blood collection vessel is clean, dry, undamaged, and compatible with the testing equipment used.

2. Add solution: Use a pipette or syringe to add the prepared lithium heparin solution to the blood collection vessel. Pay attention to controlling the amount of addition, ensuring that the solution is evenly distributed at the bottom of the sampling vessel, and avoiding local concentrations that are too high or too low.

3. Marking and recording: Mark the added heparin lithium solution on the blood collection vessel, and record the time, dosage, and concentration of the solution for subsequent experimental operations and result analysis.

Matters needing attention

1. Accurate weighing: When preparing heparin lithium solution, it is necessary to ensure the accurate weighing of the raw material powder to avoid affecting the anticoagulant effect.

2. Solution quality: Dissolve and dilute with deionized water to ensure the quality and purity of the solution. Avoid using water sources containing impurities or pollutants.

3. Storage and transportation: The prepared lithium heparin solution should be stored in a dry, cool, and dark place, avoiding direct sunlight and high temperatures. During transportation, the stability of the solution should be maintained to avoid violent vibrations and temperature changes.

4. Safe operation: During the preparation and addition process, the laboratory's safety operating procedures should be strictly followed, protective equipment should be worn, and direct contact with solutions and powders should be avoided to prevent risks such as allergies or poisoning.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of heparin lithium and other blood collection additives. It is a well-known manufacturer in China that can produce a full range of blood collection additives. Currently, in addition to heparin lithium, there are also serum separation gels, coagulants, EDTA anticoagulants, silicides, etc. available for sale, which facilitates the one-stop purchase of blood collection additives by blood collection manufacturers. Moreover, reagents produced by the same manufacturer have better results when used in combination. If you are also interested in products such as blood collection additives, please click on the official website to learn more details!