What Are The Methods To Improve The Stability Of TOOS As A Chromogenic Substrate?

N-Ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline, abbreviated as TOOS, is an important chromogenic substrate with wide applications in the field of biochemical detection. Its high molar absorbance and excellent color sensitivity make it irreplaceable in both wet chemistry and dry chemistry methods. However, the stability of TOOS is crucial for the sensitivity and accuracy of detection. Therefore, improving the stability of TOOS has become an urgent problem to be solved in scientific research and production practice. Next, we will introduce in detail several methods to improve the stability of TOOS.

1, Proper storage method

Proper storage is the first step in improving TOOS stability. Due to the easy oxidation of TOOS, improper storage can directly lead to a decrease in its stability and color development ability, thereby affecting the accuracy of the detection results. To ensure the stability of TOOS, it is recommended to use solid powder form and store it in a sealed and opaque bottle at low temperature. This storage method effectively isolates air and light, preventing TOOS from being slowly oxidized by air due to long-term storage, and avoiding deterioration caused by high temperature or light. Taking Desheng's TOOS reagent as an example, this storage method is used to ensure its long-term stability.

TOOS powder

2, Add protective agent

To improve the stability of TOOS, it can be achieved by adding protective agents. Surfactants with alkyl groups containing 8-16 carbon atoms and flavonoid pigments can serve as protective agents for TOOS. However, the formula of this method is relatively complex and may interfere with the testing. Another method is to add a protective agent with stronger reducibility than TOOS, but the protective agent in this method usually contains primary amino groups, which can easily cause non-specific reactions. Some researchers have attempted to add sodium citrate, which has a reducing effect, and found that it has a certain antioxidant effect on TOOS. However, when choosing a protective agent, it is necessary to comprehensively consider its impact on TOOS stability and whether it will interfere with subsequent testing.

3, Ready to use, ready to use

To ensure the stability of TOOS and the accuracy of detection results, it is recommended to avoid long-term storage of TOOS reagents as much as possible when using them. According to Desheng's experience, TOOS reagents should be used as soon as possible after being prepared into a solution to reduce oxidation problems caused by improper storage. This not only ensures that the reagents are used in their optimal state, but also reduces waste and error generation. Therefore, in the laboratory, a comprehensive reagent management system should be established to ensure the ready to use and dispensing of TOOS reagents.

4, Optimize reaction conditions

Optimizing reaction conditions is also an important means to improve the stability of TOOS during the reaction process. For example, in the MBTH-TOOS colorimetric system, by adjusting the pH and temperature of the reaction system, the oxidase like activity of citrate capped platinum nanoparticles (Cit PtNPs) can be significantly improved, thereby enhancing the colorimetric effect of TOOS. In addition, when using TOOS for testing, the cleanliness of the reaction system should be ensured to avoid the introduction of pollutants such as dust and microorganisms. Meanwhile, the rational use of buffering agents can stabilize the pH value of the reaction system, slow down the fading rate of TOOS, and improve the stability and reproducibility of the experiment.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of TOOS and other chromogenic substrates, and has established partnerships with numerous domestic and foreign enterprises, receiving high praise. We have various online branches selling products, and offline we have large factories, professional technical teams, and advanced production equipment. We welcome users to visit!