The Effect Of PH Changes On The Color Reaction Of ADPS Substrate 82611-88-9

The basic principle of ADPS color reaction

ADPS, as a novel Trinder's reagent, undergoes coupled oxidation reaction with reactants such as hydrogen peroxide under the catalytic action of horseradish peroxidase or peroxidase, generating quinone imine compounds. This substance exhibits a significant increase in absorbance at specific wavelengths, enabling quantitative determination of the target substance.

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Taking glucose detection as an example, glucose is oxidized by glucose oxidase to produce gluconic acid and hydrogen peroxide, which then undergoes a coupled oxidation reaction with ADPS, resulting in a significant increase in absorbance at 500nm. The increased absorbance is directly proportional to the content of the detected biomolecules, making ADPS a high-quality chromogenic substrate in biochemical analyses such as blood glucose detection.

The Effect of pH Changes on ADPS Color Reaction

In chemical reactions, changes in acidity or alkalinity often have significant impacts on reaction rates, solution stability, and biological activity. However, ADPS chromogenic substrates exhibit high stability and reliability under pH changes.

The sulfonic acid groups in ADPS molecules enable them to exhibit good solubility and stability in aqueous solutions, while the aniline groups in their molecular structure serve as key sites for interaction with key substances in enzyme catalyzed reactions. This structural characteristic enables ADPS to maintain relatively stable color development performance at different pH values.

The application advantages of ADPS in biochemical analysis

Due to the high reliability and stability of ADPS chromogenic substrates under pH changes, they have many advantages in practical applications.

1. Wide pH range applicability. ADPS can be used within a wide pH range without the need to frequently adjust the acidity or alkalinity of the reaction system, thus simplifying the experimental operation process.

2. High sensitivity and accuracy. ADPS has high sensitivity and accuracy, which can achieve accurate determination of target substances. In biochemical analysis such as blood glucose testing, the concentration of glucose in the blood can be accurately determined by measuring changes in absorbance.

3. Good water solubility and stability. ADPS has good water solubility and stability, making it easy to store and use. It can be mixed with other reagents and is suitable for different types of experimental reagent systems.

4. Widely applicable fields. Due to its excellent performance, ADPS is widely used in various biochemical test kits and diagnostic tests, such as detecting indicators such as creatinine, glucose, uric acid, or cholesterol. In addition, major biochemical reagent kit manufacturers or testing instrument companies using peroxidase methods are currently purchasing and using ADPS in large quantities, and its application fields are very extensive.

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