The Role Of Chromogenic Substrate TOOS In Glycated Albumin Assay Kit (enzymatic Method)

Basic Introduction to TOOS

TOOS, also known as N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, is a novel Trinder's reagent characterized by high water solubility, sensitivity, and stability. It is widely used in clinical biochemical testing, especially in multiple testing items such as blood glucose, uric acid, and renal function.

TOOS powder

The working principle of TOOS in glycated albumin assay kit

The glycated albumin assay kit (enzymatic method) mainly consists of glycated albumin reagent and albumin reagent, among which TOOS plays a key role as a chromogenic substrate in the measurement process. Its working principle is as follows:
Glucose oxidation to generate hydrogen peroxide: Under the catalysis of glucose oxidase, glucose is oxidized to generate hydrogen peroxide.

Coupling reaction generates color products: Hydrogen peroxide undergoes coupling reaction with MBTH and TOOS under the catalysis of platinum bismuth nanoperoxidase, producing a purple product solution. As the concentration of glucose increases, the color of the solution darkens.

Colorimetric determination of glucose content: Measure the absorbance of the solution at a wavelength of 590nm. Based on the relationship between absorbance and glucose concentration, the glucose content can be accurately calculated, thereby indirectly determining the concentration of glycated albumin.

The advantages of TOOS in the determination of glycated albumin

1. Good color rendering effect and rapid reaction: After reacting with glycosylated albumin, TOOS can quickly generate stable color products, which are easy to detect and analyze. Its color reaction product is stable and not easily faded, ensuring the accuracy and reliability of the detection results.

2. High molar absorbance, suitable for micro measurement: TOOS has a high molar absorbance, allowing it to accurately measure extremely small amounts of glycosylated albumin in serum. This is of great significance for early detection and diagnosis of diabetes.

3. Improving detection accuracy and repeatability: The application of TOOS in glycated albumin assay kits has significantly improved the accuracy and repeatability of detection. The same batch of reagent kit was used to determine the same quality control sample, and the coefficient of variation of the results was low, ensuring the stability and consistency of the detection results.

The widespread application of TOOS in medical diagnosis

1. Diagnosis and management of diabetes: As an indicator reflecting the short-term blood glucose control level, the accurate determination of glycosylated albumin is of great significance for the diagnosis, condition monitoring and treatment effect evaluation of diabetes. The application of TOOS in the saccharified albumin test kit provides strong support for the accurate management of diabetes.

2. Blood glucose monitoring of special population: in some special cases, such as dialysis anemia, acute systemic disease, liver disease, diabetes complicated with pregnancy, glycosylated albumin can more accurately reflect the average blood glucose changes in the short term than glycosylated hemoglobin. The application of TOOS makes blood glucose monitoring for these special populations more accurate and reliable.

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