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Wuhan Desheng Biochemical Technology Co., Ltd
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The Key Role of Biological Buffer BICINE 150-25-4 in Serum Guanosinase Determination

Product Details

Place of Origin: hubei

Brand Name: desheng

Certification: ISO9001

Model Number: CAS 150-25-4

Payment & Shipping Terms

Minimum Order Quantity: 20kg

Price: Detailed discussion

Packaging Details: 20kg / box

Delivery Time: 5 days

Payment Terms: L/C, D/P, T/T, Western Union

Supply Ability: 5t/week

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Specifications
Highlight:

Bicine buffer

,

150-25-4

,

biological buffer

Appearance:
White Powder
Model:
Bicine
Manufacturer:
Desheng
Packing:
500g / Box
Appearance:
White Powder
Model:
Bicine
Manufacturer:
Desheng
Packing:
500g / Box
Description
The Key Role of Biological Buffer BICINE 150-25-4 in Serum Guanosinase Determination

In the field of biomedical research, precise biochemical analysis is the key to revealing the mysteries of life, diagnosing diseases, and developing new drugs. Among them, the determination of serum guanosinase (a key enzyme involved in purine metabolism) is of great significance for understanding human metabolic processes and monitoring related disease states. To ensure the accuracy and reliability of this measurement, it is crucial to select appropriate biological buffering agents. N. N-dihydroxyethylglycine, commonly known as BICINE buffer, plays an indispensable role in serum guanine enzyme assays as an efficient and stable biological buffer.

 

Product Name N. N-dihydroxyethylglycine, BICINE buffer
CAS 150-25-4
Molecular weight 163.17
Storage conditions Room temperature, away from light and moisture
Manufacturer Hubei Xindesheng Material Technology Co., Ltd

 

The buffering performance of BICINE

 

The pKa value (acid-base dissociation constant) of BICINE is moderate and can provide effective buffering over a wide pH range, which is crucial for maintaining optimal conditions for enzyme activity in biological samples such as serum. In serum guanine enzyme determination, a stable pH environment can effectively reduce the fluctuations in enzyme activity caused by changes in acidity and alkalinity, ensuring the accuracy of the measurement results.

 

The application advantages of BICINE in serum guanine enzyme determination

 

1. Improving measurement accuracy: The activity of guanosinase in serum is susceptible to various factors, including temperature, pH value, and ionic strength. BICINE, as a buffering agent, effectively reduces the interference of external factors by precisely controlling the pH value of the reaction system, thereby improving the accuracy and repeatability of the measurement.

 

2. Protecting enzyme activity: BICINE not only acts as a buffer, but its mild chemical properties can also to some extent protect enzyme molecules from adverse external conditions such as extreme pH, metal ions, etc., ensuring that serum guanine enzymes maintain their natural conformation and activity during the measurement process.

 

3. Optimizing reaction kinetics: A suitable buffer system can promote the smooth progress of enzymatic reactions. BICINE optimizes the binding efficiency between enzymes and substrates by adjusting the ion strength and charge distribution in the solution, accelerating the reaction rate and making the activity determination of serum guanine enzymes more efficient and fast.

 

Precautions in practical operation

 

1. Concentration selection: When conducting serum guanine enzyme assays, the concentration of BICINE needs to be optimized based on specific experimental conditions and enzyme characteristics. Generally speaking, low concentrations may not provide sufficient buffering capacity, while high concentrations may affect enzyme activity. Therefore, determining the optimal BICINE concentration through preliminary experiments is a key step in ensuring successful measurement.

 

2. PH adjustment: Although BICINE has a wide buffering range, it still needs to be precisely adjusted to the optimal pH value for serum guanine enzymes in practical operation. This usually involves using a precision pH meter for monitoring and adjustment to ensure that the reaction system is in optimal condition.

 

3. Interference factor control: Although BICINE can effectively reduce some interference, there may still be other factors that affect enzyme activity in complex biological samples, such as inhibitors, activators, or interference from coexisting enzymes. Therefore, in the measurement process, comprehensive consideration and corresponding measures should be taken, such as sample pretreatment, inhibitor screening, etc., to minimize the impact of these factors.

 

Bicine and other biological buffering agents produced by Hubei Xindesheng Material Technology Co., Ltd. have high purity and good water solubility. Each batch of goods has relevant quality analysis reports to ensure product quality. Desheng has a professional technical team and quality inspectors who can provide excellent technical support and after-sales service. If you have any purchasing needs in the near future, please feel free to contact me or check the official website of Desheng!

 

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