why is it necessary to use analytical grade capso 73463-39-5 in protein sequencing and identification experiments

In the cutting-edge scientific field of protein sequencing and identification, the accuracy and reliability of experiments are crucial. 3- (cyclohexylamine) -2-hydroxy-1-propanesulfonic acid (CAPSO buffer), as a high-performance biological buffering agent, has become an indispensable component in this field due to its unique physicochemical properties. In practical applications, CAPSO at the analytical grade plays an irreplaceable role.

Basic characteristics and advantages of CAPSO

CAPSO has good water solubility and pH buffering ability, and can maintain stable pH values of solutions over a wide pH range. This characteristic is crucial for maintaining the natural conformation of proteins and preventing their denaturation. The structure and function of proteins are highly dependent on their precise three-dimensional conformation, and any small pH change can lead to protein denaturation, thereby affecting the accuracy of experimental results. In addition, CAPSO's low toxicity, low osmotic pressure characteristics, and compatibility with multiple enzymes make it an ideal biological buffer for protein sequencing and identification experiments.

CAPSO powder

Key roles in protein sequencing and identification processes

CAPSO plays a crucial role in multiple stages of protein sequencing and identification. In the protein acquisition stage, optimizing the buffer system with CAPSO can efficiently and cleanly separate target proteins, reduce non-specific binding, and improve purification efficiency. In protein electrophoresis, CAPSO, as a part of the electrophoresis buffer with good buffering performance and low conductivity, can stabilize the electric field, reduce thermal effects and protein aggregation, improve resolution and clarity, clearly distinguish proteins with small molecular weight differences, and provide reliable basis for subsequent analysis.

As the gold standard for protein sequencing and identification, mass spectrometry technology can adjust the pH of sample solutions to the optimal range, promote complete dissociation and ionization of proteins, and improve the sensitivity and accuracy of mass spectrometry detection. Its low background interference characteristics ensure clear mass spectrometry spectra, facilitating data analysis and protein sequence inference. In structural biology research, CAPSO, as a stabilizer or additive, can maintain stable protein conformation, prevent phase transition or degradation during crystallization, and assist in high-resolution protein structure analysis.

The necessity of analyzing pure level CAPSO

CAPSO of analytical grade has higher purity and stricter quality control standards. In protein sequencing and identification experiments, any impurities may interfere with the experimental results, leading to inaccurate data. Analyzing pure grade CAPSO can minimize the impact of impurities and ensure the reliability and reproducibility of experimental results. For example, in mass spectrometry analysis, if the buffer contains impurities, it may generate additional signals that interfere with the detection and sequence inference of target proteins. The use of analytical grade CAPSO can avoid this situation and improve the success rate of experiments and the accuracy of data.

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In addition to CAPSO buffer, Desheng also produces other high-quality biological buffer products, such as tris besie, tes free acid, and dozens of other products. These products also have good performance and reliable quality, which can meet the needs of different experiments. At present, Desheng has established cooperation with hundreds of domestic and foreign enterprises, which has been widely recognized by customers. This is also a manifestation of the good competitiveness of Desheng products! If you have any recent purchasing needs, please click on the official website of Desheng to learn more details!