Analysis Of The Principle , Process , And Advantages Of HDAOS 82692-88-4 Detection Of Serum Total Cholesterol

In the field of clinical biochemical testing, accurate detection of serum total cholesterol is of great significance for the diagnosis, disease monitoring, and risk assessment of various diseases such as cardiovascular disease and liver disease. The new Trinder's reagent, as a commonly used class of chromogenic substrates in biochemical reagents, plays a key role in the detection of biochemical indicators, based on enzyme catalyzed spectrophotometry. Different Trinder's reagents exhibit certain differences in detecting different biochemical indicators. This article will delve into the specific application of HDAOS in detecting serum total cholesterol levels.

1, Sample collection and processing

Before using HDAOS to detect serum total cholesterol, sample collection and processing are crucial steps in the entire detection process. Normally, a vacuum blood collection vessel anticoagulant tube is used to collect venous blood, and the test sample can be either serum or plasma. If anticoagulant samples are selected for testing, they should be gently inverted and mixed 5-8 times after collection to ensure sufficient contact between the sample and anticoagulant, prevent blood clotting, and ensure sample quality, providing a reliable basis for subsequent testing.

HDAOS Powder

2, The principle and process of detecting cholesterol in HDAOS samples

Cholesterol, also known as cholesterol, is a derivative of cyclopentane dihydrophenanthrene. The total cholesterol in serum consists of two parts: free cholesterol and cholesterol esters. During the HDAOS detection process, cholesterol esters in the serum first undergo hydrolysis reaction under the action of cholesterol esterase, generating free cholesterol and free fatty acids. Subsequently, cholesterol is further converted into 4-cholesterenone and hydrogen peroxide through the catalytic oxidation of cholesterol oxidase. When peroxidase (such as HRP or POD) is present, the generated hydrogen peroxide promotes the coupling oxidation reaction between the chromogenic substrate HDAOS and 4-aminoantipyrine, ultimately producing red quinone imine compounds. This red quinone imine substance has characteristic absorbance at a specific wavelength, and its absorbance value is detected by a spectrophotometer. As the absorbance is directly proportional to the total cholesterol (TC) content in the sample, the total cholesterol content in the sample can be accurately calculated based on a pre drawn standard curve.

3, Advantages of HDAOS cholesterol detection

There are over a hundred methods for determining serum total cholesterol, but the enzymatic method using the novel Trinder's reagent HDAOS is currently the most commonly used method with many significant advantages. Firstly, this method has fast detection speed and accurate results, and can obtain reliable detection results in a short period of time, meeting the needs of rapid clinical diagnosis. Secondly, it has strong specificity and can specifically detect cholesterol, effectively avoiding interference from other substances and improving the accuracy of detection. In addition, this detection method is easy to operate and does not require complex extraction steps, making it convenient for automated analysis and batch determination, greatly improving detection efficiency.

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Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of new trinder's reagents. Currently, in addition to HDAOS powder, the new trinder's reagents on sale also include TOOS, TOPS, ADOS, ADPS, ALPS, ALPS, DAOS, MADB, MAOS, etc. Desheng has a professional technical team to ensure product quality while providing customized services and excellent after-sales service. If you have any recent purchasing needs, please click on the official website to learn more details or contact me!