DIPSO buffer 68399-80-4: a stable guardian for chromatographic separation and electrophoresis technology

In biochemical and molecular biology experiments, even small fluctuations in solution pH can affect the accuracy of experimental results. As an efficient biological buffer, 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid (DIPSO) has become an indispensable stabilizer in chromatographic separation and electrophoresis technology due to its unique chemical properties and wide range of application scenarios. This article will analyze how DIPSO provides precise pH control support for experiments based on its molecular characteristics, technical principles, and practical applications.

1, Molecular properties: buffering advantage of dihydroxyethylamine

The molecular structure of DIPSO contains two hydroxyethyl amino groups and one sulfonic acid group, and this dipole ion property gives it excellent buffering ability in the pH range of 7.0 to 8.2. Its pKa value (7.4-7.8 at 25 ℃) is close to the physiological environment and can quickly respond to external acid-base changes, maintaining the dynamic balance of solution pH.

In addition, the chemical inertness of DIPSO makes it almost insoluble in organic solvents, which is particularly important in chromatographic separation. When used for protein purification, DIPSO can avoid non-specific binding with target molecules and optimize separation efficiency by adjusting the pH value of the mobile phase.

2, Chromatographic separation: precise pH regulation to improve separation efficiency

In chromatographic technology, pH directly affects the ionization state of target molecules and the surface charge of the stationary phase, thereby determining the separation efficiency. The buffering capacity of DIPSO makes it an ideal choice for separating biomolecules such as proteins and nucleic acids. For example, in ion exchange chromatography, by adjusting the pH value of DIPSO buffer, the net charge of the target molecule can be controlled, achieving gradient elution of proteins with different isoelectric points.

3, Electrophoretic technology: stabilize pH gradient to ensure migration accuracy

In electrophoresis experiments, the pH value of the solution directly affects the migration rate and direction of charged molecules. The buffering capacity of DIPSO ensures a constant pH value during electrophoresis, avoiding local pH changes caused by Joule heating generated by current. For example, in polyacrylamide gel electrophoresis (SDS-PAGE), DIPSO buffer can maintain the stability of gel pore size and ensure the accurate separation of protein molecules according to molecular weight. Experimental comparison shows that when using DIPSO buffer system, the migration distance error of protein bands can be controlled within ± 1%.

In isoelectric focusing electrophoresis (IEF), the wide pH buffer range (7.0-8.2) of DIPSO can cover the isoelectric point region of most proteins, achieving high-resolution separation. Its low ionic strength characteristic can also reduce electric field distortion and further improve separation efficiency. For example, in clinical diagnosis, DIPSO buffer has been used for isoelectric focusing analysis of serum proteins, helping doctors accurately identify abnormal protein peaks.

As a supplier of biological buffering agents such as DIPSO, Hubei Xindesheng ensures that every batch of DIPSO buffering agents has high purity and stable performance. New Desheng can meet the requirements in various fields, ensuring timely and accurate delivery of products to customers with sufficient inventory and fast logistics distribution. If you are interested, please feel free to click on the website for consultation at any time!