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Biological buffer Tris HCl powder 1185-53-1 preservation guide: frozen or room temperature?

Product Details

Place of Origin: hubei

Brand Name: desheng

Certification: ISO9000

Model Number: CAS 1185-53-1

Payment & Shipping Terms

Minimum Order Quantity: 20kg

Price: Detailed discussion

Packaging Details: 20kg / box

Delivery Time: 5 days

Payment Terms: L/C,D/P,T/T,Western Union

Supply Ability: 5t/week

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Specifications
Highlight:

Analytical reagent

,

High purity reagent

,

Good water solubility

Appearance:
White Powder
Model:
TRIS HCL
Manufacturer:
Desheng
Packing:
500g / Box
Appearance:
White Powder
Model:
TRIS HCL
Manufacturer:
Desheng
Packing:
500g / Box
Description
Biological buffer Tris HCl powder 1185-53-1 preservation guide: frozen or room temperature?

In biochemical laboratories, Tris HCl, a core buffering agent, is widely used in protein research, nucleic acid extraction, and cell culture due to its excellent pH regulation ability. However, how to properly preserve this' stability guard 'and ensure its performance is not affected by the environment has become a focus of concern for many researchers. This article will start from scientific principles and combine with practical application scenarios to analyze the key points of Tris HCl powder preservation, helping you easily cope with experimental challenges.


Product name TRIS HYDROCHLORIDE
chemical abbreviation
Tris HCl buffer
Product appearance White crystalline powder CAS NO 1185-53-1
Molecular weight 157.6 Product advantages Purity ≥ 99%, good water solubility, stable process
Molecular formula C4H12ClNO3 Storage conditions Room temperature, away from light and moisture
Application Used for biological buffer solution, adjusting pH value
Manufacturer Hubei Xindesheng Material Technology Co., Ltd


1, The chemical properties of Tris HCl: why stability is crucial?


Tris HCl is formed by neutralizing Tris with hydrochloric acid, and its buffering ability comes from the dynamic equilibrium between the amino group (- NH ₂) of Tris molecule and hydrogen ions in solution. This characteristic makes it perform well in the pH range of 7.0-9.0, but also imposes strict requirements on storage conditions.


Biological buffer Tris HCl powder 1185-53-1 preservation guide: frozen or room temperature? 0

Tris HCl powder


Moisture absorption: Tris molecules contain multiple hydroxyl groups (- OH), which can easily absorb moisture when exposed to air, leading to powder clumping and even degradation.


PH sensitivity: Fluctuations in environmental humidity or temperature may trigger minor hydrolysis reactions, affecting the final pH value of the buffer solution.

Light and oxidation risks: Long term exposure to light or metal ions may accelerate Tris oxidation, generating by-products that affect experimental results.

Therefore, the core goal of scientifically preserving Tris HCl powder is to isolate moisture, control temperature, and reduce external pollution.


2, Freezing preservation: necessary or excessive protection?


Regarding whether Tris HCl needs to be frozen, a comprehensive judgment should be made based on its chemical stability and experimental requirements

Short term use: If the laboratory environment is dry (relative humidity<40%) and the temperature is stable (15-25 ℃), seal Tris HCl in a moisture-proof bottle and place it in a cool and dark place to meet the requirements. At this point, freezing may cause powder clumping due to repeated freeze-thaw cycles, increasing the difficulty of weighing.


Special scenario: If the powder has slightly clumped, it can be re ground and used after low-temperature drying in a drying oven, but its pH buffering capacity needs to be verified to meet the standard.


3, The Four Golden Rules for Optimizing Preservation


Following the following principles can maximize the lifespan of Tris HCl:


Sealed and moisture-proof: Use sealed bottles with desiccants or store after vacuum packaging to avoid direct contact with air.


Avoid light storage: Ultraviolet radiation may induce oxidation of Tris HCl molecules, and the container should be wrapped in brown bottles or aluminum foil.

Independent packaging: Divide the powder according to the experimental dosage to reduce the risk of contamination caused by repeated bottle opening.

Regular testing: Test the pH value of the buffer solution every 6 months to ensure it meets the experimental requirements.


Biological buffer Tris HCl powder 1185-53-1 preservation guide: frozen or room temperature? 1

Product packaging


Hubei Xindesheng Material Technology Co., Ltd. has been specializing in the research and production of biological buffering agents for nearly 20 years. We have unique insights into the selection of raw materials, updates in production technology, product quality testing, and control of production costs. We are committed to providing customers with affordable and high-quality products! If you have any purchasing needs in the near future, please contact me immediately!

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