More precise separation, clearer stripes! BIS-TRIS: The 'key to stability' for your electrophoresis experiment

In contemporary molecular biology research, electrophoresis technology is undoubtedly one of the core methods for separating and analyzing biomolecules. In this delicate art of separation, the choice of buffer system directly determines the success or failure of the experiment. Among numerous buffering reagents, bis (2-hydroxyethyl) amino (trihydroxymethyl) methane (BIS-TRIS) has become an indispensable and important reagent in electrophoresis experiments due to its unique chemical properties and excellent buffering performance. This article will delve into the core applications of BIS-TRIS in electrophoresis experiments, revealing its irreplaceable value to you.

The core application of BIS-TRIS in protein electrophoresis

In protein electrophoresis, especially in isoelectric focusing (IEF) and two-dimensional electrophoresis (2-DE) experiments, the stability of pH gradient is crucial. BIS-TRIS, with its pKa value of 6.5, precisely fills the pH gap between the phosphate buffer system and the Tris buffer system, providing a smoother and more stable pH gradient for protein separation.

BIS-TRIS Powder

More importantly, the weak binding ability of BIS-TRIS to metal ions effectively reduces the interference of metal ions on protein migration, ensuring the accuracy and repeatability of protein separation. This advantage enables researchers to obtain clearer and more accurate protein separation results, laying a solid foundation for subsequent protein identification and functional research.

The unique value of BIS-TRIS in nucleic acid electrophoresis

In nucleic acid electrophoresis experiments, especially RNA electrophoresis, preventing nucleic acid degradation is the key to the success of the experiment. The BIS-TRIS buffer system can provide a mild separation environment and effectively reduce the risk of RNA hydrolysis. Meanwhile, its excellent buffer capacity ensures the stability of pH value during long-term electrophoresis, providing consistent environmental conditions for nucleic acid separation.

Compared with traditional buffer solutions, the BIS-TRIS buffer system can provide clearer nucleic acid bands and higher resolution, especially when separating small molecule nucleic acid fragments. This advantage makes BIS-TRIS the preferred buffer reagent for high-end nucleic acid electrophoresis experiments.

Why choose BIS-TRIS buffer

When choosing electrophoresis buffer reagents, researchers usually compare multiple options. BIS-TRIS stands out due to the following major advantages:

1. Wider buffering range: BIS-TRIS has excellent buffering ability in the pH range of 5.8-7.2, perfectly covering the isoelectric point range of many biomolecules.

2. Low metal ion binding force: This characteristic reduces the interference of metal ions on electrophoretic mobility, improving the accuracy and reproducibility of separation results.

3. High solubility: BIS-TRIS has extremely high solubility in water, making it easy to prepare high concentration reserve solutions and convenient to use.

4. Low temperature sensitivity: Its pKa value changes little with temperature, providing stability guarantee for experiments under different temperature conditions.

Product packaging

In summary, bis (2-hydroxyethyl) amino (trihydroxymethyl) methane (BIS-TRIS) has become an irreplaceable key reagent in modern electrophoresis experiments due to its unique chemical properties and excellent buffering performance. Whether it is protein or nucleic acid electrophoresis, BIS-TRIS can provide a stable and reliable buffering environment, ensuring high resolution and reproducibility of separation results.

To obtain accurate electrophoresis experimental data, in addition to selecting suitable biological buffering agents, it is also necessary to cooperate with reliable biological buffering agent suppliers. Hubei Xindesheng Material Technology Co., Ltd. has rich R&D and production experience in the production of biological buffering agents and can provide good customized and after-sales services. If interested, please feel free to contact me!