Cas 77-86-1 PH 7.2 PH 7.3 Tris Buffer Solution Interpret The Buffer Range

Interpret the buffer range and application of Tris in detail,Cas 77-86-1 PH 7.2 PH7.3

The full name of Tris is trihydromethylaminomethane, which is one of the biological buffers. Appearance is white crystalline powder, good water solubility, soluble in ethanol, acetic acid, ethyl ester, but insoluble in carbon tetrachloride. Tris can be configured as a solution. Tris buffer is a kind of solution that can resist the influence of a small amount of strong acid and alkali from the outside and maintain the Ph value of the system unchanged. Tris buffer is very important in the research of biochemistry.

Tris buffer range:

Tris is a weak base, and its pKa value is 8.1 at room temperature 25°C. According to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2, and the pH of a 0.1 mol/l aqueous solution is 10.4. Generally, hydrochloric acid is added to adjust the pH value to obtain a buffer with the required pH value. But at the same time, it is necessary to pay attention to the influence of temperature on its pKa and affect the Tris buffer. Every time the temperature decreases by 1℃, the pH increases by about 0.03 units, and the concentration is diluted by 10 times, and the pH decreases by 0.03-0.05 units. And Tris buffer is very easy to absorb CO2 in the air, so the prepared buffer needs to be sealed. In addition, the Tris buffer will have a certain interference effect on some pH electrodes, so an electrode compatible with the Tris buffer should be used when using it.

Application of Tris in buffer:

1. 1M Tris-HCL6.8 and 1.5M Tris-HCL8.8 and 5xTris-glycine running buffer are the most commonly used reagents for SDS-PAGE.

2. Add EDTA to Tris hydrochloric acid buffer to make TE buffer. TE buffer is used for DNA stabilization and storage. Changing the pH value of hydrochloric acid to acetic acid can obtain TAE buffer, and changing to boric acid to obtain TBE buffer, and both TAE and TBE buffers are commonly used in nucleic acid electrophoresis experiments.

3. Tris is used for protein crystal growth under different pH conditions.

4. The low ionic strength of Tris buffer can be used to form the intermediate fibers of the nematode nuclear laminin.

Therefore, Tris buffer is a solvent for nucleic acids and proteins, and is widely used in the preparation of buffers in biochemistry and molecular biology experiments.

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