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|CAS:||77-86-1||Appearance:||White Crystal Powder|
Tris Base Hydroxymethylaminomethane,
CAS77-86-1 Saturated Phenol
Detailed configuration steps of tris(hydroxymethylaminomethane) (CAS77-86-1)saturated phenol,tris base supplier
The full name of Tris: Tris, as a common chemical product, has a very wide range of applications in various fields. For example, Tris can be seen in the fields of medicine, daily chemicals, special materials, etc., and there is an application in it, "nucleic acid purification". Nucleic acid purification requires saturated phenols. Tris also has saturated phenols, also known as alkaline phenols and Tris balanced phenols.
Tris saturated phenol is redistilled phenol saturated with Tris-ClpH8.0. It is a light yellow transparent liquid with pungent odor. The upper layer is Tris hydrochloric acid buffer, and the solution is added with antioxidant 8-hydroxyquinoline. It is suitable for removing protein from nucleic acid samples, extracting DNA, etc. Although there are ready-made products on the market, it is better to master the configuration method of Tris saturated phenol. The detailed configuration steps of Tris saturated phenol will be introduced below.
Tris saturated phenol:
|1||Take out the re-vaporized phenol from the refrigerator and place it at room temperature --- it melts in a 68-degree water bath. Do not put it in a 68-degree water bath immediately to prevent the glass from bursting;|
|2||Add 8-hydroxyquinoline to 0.1% and β-mercaptoethanol to 0.2%, (stock solution 14. 4MOL/ML), mix well, the solution turns yellow, pour it into a separatory funnel (can also be done in a beaker) ；|
|3||Add 1MOL/ML of Tris (PH8.0) of equal solvent, mix repeatedly, and let it stand until it separates; release the yellow phenol solution of the lower layer, and discard the upper layer;|
|4||Add solid Tris about 1g/100ml phenol, shake well, and remove the water phase;|
|5||Add 0.1MOL/MLTris (PH8.0) and balance several times until the pH is 8.0;|
|6||Add 0.1MOL/MLTris (PH8.0) and store in a brown bottle at 4°C;|
|7||If the yellow color disappears or turns pink, it cannot be used.|
Tris saturated phenol is toxic and has a strong irritating effect on the skin and human body. It needs to be operated in a fume hood. Avoid hazards, safety first.
The above is the configuration method of Tris saturated phenol provided by Hubei Xindesheng Material Co., Ltd. In addition to Tris, Desheng also produces other chemicals, such as blood collection tube additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates, and carbomer products. At present, Desheng company has been established for decades, has its own R&D team, and has invested a lot of energy in biological buffers. The quality of the products is reliable, the price is favorable, and the price can be discussed. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
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