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|Molecular Weight:||121.135||Molecular Formula:||C4H11NO3|
|Appearance:||White Crystal Powder||Storage Conditions:||Room Temperature, Light-proof And Moisture-proof|
99% Tris Hydroxymethyl Aminoethane,
CAS 77-86-1 Tris Hydroxymethyl Aminoethane,
Tris Base 77-86-1
99% Tris(Hydroxymethyl)Aminoethane Tris Base 77-86-1 use for Biological buffer
All you want to know about TRIS buffer is here
1. What is TRIS buffer?
Tris(hydroxymethyl)aminomethane, or TRIS for short, is called tromethamine or THAM in medical applications. It is an organic compound with the molecular formula (HOCH2)3CNH2. It is widely used in biochemistry and molecular biology as a component of buffer solutions, and its derivatives include TAE and TBE buffers.
TRIS buffer powder
|PH value required(25℃)||7.10||7.20||7.30||7.40||7.50||7.60||7.70||7.80||7.90||8.00||8.10||8.20||8.30||8.40||8.50||8.60||8.70||8.80||8.90|
|0.1mol/L HCI volume||45.7||44.7||43.4||42||40.3||38.5||36.6||34.5||32||29.2||26.2||22.9||19.9||17.2||14.7||12.4||10.3||8.5||7|
2. What is the pH value of TRIS buffer?
Tris buffer is an ideal choice for most biological systems because its pKa value at 25°C is about 8.1 making it an effective buffer in the pH range of 7-9. This pH range is suitable for most biological experiments. This is also the reason why TRIS buffer is widely used.
3. Are Tris buffer and Tris base the same?
The pH value of Tris-base 1M is about 11, and the pH value can be reduced to 7 by adding HCL. But in Tris-Hcl, if you want to lower the pH to 7 you must add NaOH, because tris-Hcl contains hydrochloric acid, so it has a higher ionic strength (HCl + NaOH). But in Tris-base, you only need to add HCl.
4. Why use TRIS for DNA extraction?
Tris is a common biological buffer that can be used in the entire DNA extraction process. Because it is sensitive to pH during DNA extraction. Therefore, in the process of cell lysis, removal of unwanted cell components and precipitation, tris buffer is needed to maintain a stable pH value.
5. What is the difference between TAE and TBE?
TAE buffer is a solution composed of Tris base, acetic acid and EDTA. TBE buffer is a solution composed of Tris base, boric acid and EDTA. TAE has no effect on subsequent experiments, but the resolution is relatively low. The presence of boron ions in TBE will slightly affect the activity of some enzymes, but the resolution is high. Because TAE contains acetic acid, the buffering capacity is not as good as that of TBE.
Desheng Company is a manufacturer specializing in the production of biological buffers, and has been committed to the development and preparation of biological buffers for more than ten years. The production experience is very rich, and the pre-sales, in-sales and after-sales can bring you an unprecedented shopping experience. If necessary, please feel free to contact us
Tris has a certain interference effect on some PH electrodes, and it is necessary to use electrodes that are compatible with Tris solution. In addition, Tris is not suitable for the determination of bicinchoninic acid (BCA), and Tris needs to wear gloves and goggles during operation, because Tris is inhaled, ingestion and skin absorption have certain effects on the human body.
Desheng Biotechnology has been developing and producing biological buffers for many years, and has invested a lot of funds in the development and production of Tris series products. At present, the company's products have been sold to more than 100 countries around the world. The quality of the products is reliable, and the price can be negotiated. If you are interested, you can call for consultation. Desheng Biotechnology welcomes your calls.
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