Method for assay of heparin sodium content used for blood anticoagulation

Heparin sodium is a commonly used blood anticoagulant reagent, which is widely used in clinical medical fields. To ensure its quality and purity, accurate and reliable determination is crucial. Below, we will introduce the importance of its measurement and the ways in which it can be efficiently and accurately measured.

Heparin sodium parameters:

The Importance of Heparin Sodium Determination

1. Ensure safe use: As a blood processing reagent, although it does not come into direct contact with the human body, it is necessary to ensure that it does not cause harm and can react accurately after contact with blood. Measuring heparin sodium can ensure accurate dosage and avoid the impact of high or low concentration.

2. Quality control: The manufacturer controls the quality of heparin sodium to ensure that the product meets the specified quality standards, which can ensure the stability and effectiveness of use.

3. Research and development: Scientific researchers accurately measure in their product research, which is conducive to providing relatively accurate scientific basis for the use of blood vessels in clinical practice.

Heparin sodium powder

Method for determination of heparin sodium

1. Determination of adsorption rate

After a certain interval of time, the adsorption potency is tested to calculate the total residual potency in the solution. This allows the total residual potency to characterize the adsorption capacity of the resin. Measure the potency of heparin sodium solution before adsorption, set the potency value to D0 before adsorption, and measure the potency of the experimental solution after adsorption. Set the potency value to D1 after adsorption, and take the average of three parallel experiments.

2. Determination of absorbance

Generally, a UV spectrophotometer is used for measurement. When monochromatic light radiation passes through the solution of the substance being measured, the amount absorbed by the substance is proportional to the thickness and concentration of the liquid layer of the substance.

3. Determination of solution concentration

Prepare the sample solution and backup solution separately, with the amount of the tested component contained in the backup solution being 100% ± 10%, but the reagents used should be completely consistent. After measuring the absorbance of the sample solution and backup solution at the specified wavelength, calculate the concentration of the tested solution in the test sample according to the formula.

4. Determination of potency

Usually, the sheep plasma method is used for measurement. A water bath is prepared at 37 ℃ for constant temperature, frozen sheep plasma is taken out, melted, and filtered for use. Then, two sets of test tubes with different specifications are prepared, and the same amount of sodium citrate solution, calcium chloride, and filtered sheep plasma are added to the test tubes. Seal the test tube, invert it 3-4 times, mix thoroughly, and then place it vertically in a constant temperature water bath for 1 hour. After 1 hour, check the degree of condensation and record the results.

The measurement method mentioned above is efficient, fast, accurate, and reliable, which is simpler than other operational measurement methods and reduces the operational burden on experimental personnel. Desheng is a professional manufacturer of heparin sodium, providing customers with reliable measurement methods and technical guidance. The products produced have been widely used in blood collection and have stable performance. If you are interested, please click on the website to inquire and purchase!