How To Determine Whether The Chromogenic Substrate DAPS 82611-88-9 Has Deteriorated

Ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium salt, abbreviated as ADPS reagent, is an important chromogenic substrate with wide applications in the field of biochemistry. Its high water solubility, sensitivity, and stability make it a key component in biochemical test kits, commonly used for detecting indicators such as blood glucose, creatinine, uric acid, cholesterol, etc. However, the storage and usage conditions of ADPS have a significant impact on its stability, so it is particularly important to understand how to determine whether ADPS has deteriorated.

Basic characteristics of ADPS

ADPS, Also known as ESPAS or N-ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium dihydrate, it is a highly water-soluble derivative of aniline. Its appearance is white crystalline powder, with a purity of over 99%, a pH range of 5.5-9.5, and an absorption wavelength of up to 540nm.

ADPS powder

ADPS undergoes coupled oxidation reaction with reactants such as hydrogen peroxide (H2O2) under the catalytic action of horseradish peroxidase (HRP) or peroxidase (POD), generating quinone imine compounds. These compounds exhibit a significant increase in absorbance at specific wavelengths (such as 500nm), enabling quantitative determination of target substances.

Method for determining whether ADPS has deteriorated

1. Observe the appearance. ADPS with fresh dates and good quality should be in the form of white crystalline powder. If there is a significant change in color, or if there is clumping, moisture absorption, or mold growth, it can be preliminarily judged that ADPS has deteriorated.

2. Detect solubility. ADPS should have good solubility in aqueous solution. If the dissolution rate slows down or cannot be completely dissolved, it may be due to a change in its chemical structure.

3. Measure absorbance. Measure the absorbance value of ADPS coupled with reactants after oxidation at a specific wavelength using spectrophotometry. If the absorbance value significantly decreases, it indicates that the color development ability of ADPS has decreased.

4. PH value test. Observe whether the color reaction of ADPS is stable at different pH values. If pH fluctuations have a significant impact on the color reaction, it may be a manifestation of ADPS deterioration.

5. Chemical reagent testing. By conducting comparative experiments with standard chemical reagents, observe whether the color development effect of ADPS is consistent. If the difference is significant, it may be that ADPS has deteriorated.

6. Storage time check. Check the purchase date and storage time of ADPS. ADPS stored for a long time, especially under poor storage conditions, is more prone to deterioration.

In summary, to determine whether the chromogenic substrate N-ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium salt ADPS has deteriorated, it is necessary to consider multiple aspects comprehensively. Firstly, it is necessary to understand the basic characteristics of ADPS reagents. By observing changes in appearance and solubility, checking the expiration date and storage time, preliminary judgments can be made. In order to make the judgment results more accurate, the performance of ADPS reagents can be tested. Only by ensuring the quality and stability of the reagents can accurate and reliable experimental results be obtained.

Product packaging

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