Potential Risk Analysis of Expired Use of ADPS Powder 82611-88-9

ADPS, as the new Trinder's reagent, occupies an important position in the field of biochemical detection due to its high water solubility and chemical stability. The core mechanism of its action is that ADPS undergoes coupled oxidation reaction with hydrogen peroxide under the catalysis of horseradish peroxidase (HRP) or peroxidase (POD), generating quinone imine compounds with characteristic absorbance. This characteristic makes it widely used in biochemical indicators such as blood glucose, blood lipids, renal function detection, as well as experimental scenarios such as catalase activity determination.

Risk of deterioration of expired ADPS powder

1. Appearance and solubility changes

Fresh ADPS is a pure white crystalline powder. If it turns yellow, clumps, or absorbs moisture, it indicates that its chemical structure has changed. Reduced solubility is an important indicator of deterioration, such as slower dissolution rate or incomplete dissolution, which may be due to intermolecular cross-linking or impurity accumulation. Such changes directly affect the efficiency of the color reaction, resulting in detection results deviating from the true values.

ADPS powder

2. Decreased color rendering ability

By spectrophotometric detection, the absorbance value of expired ADPS significantly decreased at a wavelength of 500nm. For example, after normal ADPS reacts with hydrogen peroxide, the absorbance should increase linearly with substrate concentration, while expired samples may have insufficient color intensity or nonlinear response due to oxidation or degradation of active groups.

3. PH stability disruption

The color reaction of ADPS is highly sensitive to pH, and its applicable range is 5.5-9.5. Expired samples may accumulate acidic or alkaline impurities, leading to a decrease in the tolerance of color reactions to pH changes.

The impact of expired ADPS powder on experimental results

1. Decreased detection sensitivity

In the determination of catalase activity, the absorbance change of ADPS directly reflects the enzyme activity. If expired ADPS is used, its molar absorptivity may significantly decrease, leading to inaccurate detection of low concentration samples and misjudgment as "enzyme activity deficiency".

2. Risk of false positives or false negatives

The color reaction of expired ADPS may exhibit non specificity, such as producing background absorbance in the absence of hydrogen peroxide, leading to false positive results; Or due to reduced color rendering efficiency, it may not respond effectively to low concentration samples, leading to false negatives.

3. Poor repeatability between batches

The laboratory quality control requires small differences between reagent batches, and the chemical composition of expired ADPS may undergo uneven changes, such as some molecules degrading into inactive products, resulting in significant differences in color development efficiency among different packaging within the same batch, seriously affecting the reproducibility of experiments.

Safety hazards of expired ADPS powder

1. Chemical stability risk

The sulfonic acid group of ADPS are prone to hydrolysis in high temperature or humid environments. If the laboratory does not strictly follow the low-temperature and dark storage conditions, expired samples may release hazardous substances, thereby affecting the health of laboratory personnel.

2. Risk of waste disposal

The chemical structural changes of expired ADPS may lead to a decrease in its biodegradability, and if directly discharged into the sewer, it may have long-term impacts on the aquatic ecosystem. In addition, the accumulation of its sodium salt component in the soil may alter the local pH value and affect microbial activity.

Product packaging

The expired use of ADPS powder not only leads to distortion of experimental data, but also may cause safety hazards. Therefore, when purchasing and using ADPS powder, it is important to pay attention to the production date and shelf life, and scientifically handle expired ADPS powder. Hubei Xindesheng Material Technology Co., Ltd. specializes in producing new Trinder's reagents such as ADPS. If you have any related needs, please feel free to contact me at any time!