China Wuhan Desheng Biochemical Technology Co., Ltd Company News

In the field of modern biomedical research and clinical diagnosis, chemiluminescence immunoassay (CLIA) has become an indispensable technology for detecting various biomolecules with high sensitivity and specificity. Among them, Luminol and Horseradish Peroxidase (HRP) are common participants in this technology. Although they remain independent in physical space, they exhibit exquisite cooperation in chemiluminescence reactions. 1、 Luminol: a substrate for chemiluminescence Luminol is a compound that can spontaneously emit light through chemical reactions, a phenomenon known as chemiluminescence. In chemiluminescence immunoassay, luminol is used as a substrate. When it is excited by oxidants (such as hydrogen peroxide H ₂ O ₂) and in an appropriate alkaline environment, it can generate photons and emit visible light. This process not only provides intuitive signals for detection, but also is a key step in signal amplification in chemiluminescence immunoassay. 2、 HRP: The driving force behind catalytic reactions HRP is an enzyme widely used in immunoassays, which has the ability to catalyze specific chemical reactions. In chemiluminescence immunoassay, HRP is commonly used to label antibodies or other detection molecules. When the HRP labeled detection molecule binds to the target analyte (such as antigen or antibody), HRP becomes a part of the complex. At this point, the catalytic activity of HRP is activated, which can accelerate the oxidation reaction of luminol and trigger chemiluminescence. 3、 The synergistic effect of luminol and HRP Although luminol and HRP play completely different roles in chemiluminescence immunoassay, there is an inseparable relationship between them. The presence of luminol provides a stage for HRP to exert catalytic effects, and HRP catalysis is a prerequisite for luminol luminescence. Without the catalytic effect of HRP, even with sufficient luminol and oxidant, chemiluminescence will not be produced; On the contrary, without luminol as a substrate, the catalytic activity of HRP cannot be converted into observable light signals.   4、 Physical separation and functional fusion In the practical operation of chemiluminescence immunoassay, luminol and HRP are physically separated components. HRP labeled antibodies or proteins first form complexes with the target analyte in the sample, and then reagents required for chemiluminescence reactions such as luminol and hydrogen peroxide are added under specific conditions. At this point, HRP begins to catalyze the oxidation reaction of luminol, leading to the production of chemiluminescence. This process not only reflects the independence of luminol and HRP in physical space, but also demonstrates their interdependent functional fusion in chemical reactions. Luminol and HRP in chemiluminescence immunoassay, although physically separated, exhibit good cooperation in chemiluminescence reactions. This art of separation and collaboration not only reveals the basic principles of chemiluminescence immunoassay, but also provides powerful tools for biomedical researchers to detect and quantify biomolecules with high precision, promoting progress in disease diagnosis and scientific research.   Through the analysis of the roles of luminol and HRP in chemiluminescence immunoassay, we have gained insight into the intricate and complex cooperative patterns in modern biotechnology, while also deepening our understanding and application of this important detection technique. In the future, with the continuous advancement of science and technology, chemiluminescence immunoassay is expected to demonstrate its value and potential in more fields. As a manufacturer of luminescent reagents, Desheng has a complete range of luminol types, high sensitivity, and professional personnel to track and guide, helping to solve technical problems. If you have any relevant intentions, please click on the website to inquire about details!

Acridine ester, as an important chemiluminescence marker, has a wide range of applications in biological detection, medical diagnosis and other fields. However, its stability in aqueous solution, especially its hydrolysis characteristics, is one of the key factors affecting its application effectiveness. This article will provide a detailed exploration of the hydrolysis mechanism, influencing factors, and stability improvement measures of acridine esters.   1、 The hydrolysis mechanism of acridine ester The hydrolysis process of acridine ester in aqueous solution is a complex chemical reaction. When the pH value in the solution exceeds a certain range (especially under alkaline conditions), the ester bonds in the acridine ester molecules are easily attacked by OH - ions, generating a pseudo base. Subsequently, under the continued action of OH - ions, the ester bond of the pseudo base is broken, ultimately resulting in the formation of N-methylacridone. This hydrolysis process is a non luminescent dark reaction process, which can lead to a decrease in the stability of acridine ester, thereby affecting its luminescence effect and detection sensitivity. 2、 Factors affecting the hydrolysis of acridine esters PH value: pH value is one of the key factors affecting the hydrolysis of acridine esters. When the pH value of the solution is less than 4.8, the photo quantum yield of acridine ester and its protein conjugate does not decrease after being stored at room temperature for 4 weeks; Freeze dried products can be stored for more than one year at -20 ℃. However, when the pH value is greater than 4.8, especially in alkaline solutions, the stability of acridine esters will significantly decrease, and the degree of hydrolysis will increase with the increase of pH. Temperature: Temperature is also an important factor affecting the hydrolysis of acridine esters. As the temperature increases, the hydrolysis rate of acridine ester will accelerate and its stability will decrease. Therefore, it is necessary to control the appropriate temperature during storage and use to ensure the stability of acridine esters. Substituents: The type of substituent attached to the acridine ring, phenolic ring, or benzenesulfonyl ring can also affect the stability of acridine esters. Acridine esters or acridine sulfonamides with methyl or other substituents have increased thermal stability due to their high steric hindrance; When there are electron withdrawing groups attached, the stability decreases due to the favorable nucleophilic substitution reaction.   3、 Measures to improve the stability of acridine esters Control pH value: Acridine esters exhibit high stability in acidic environments (pH

Luminol solution, as a widely used reagent in the fields of chemiluminescence and forensic science, its shelf life is a matter of great concern for experimenters and researchers. Especially at room temperature, how to properly preserve luminol solution to ensure its effectiveness when needed has become a topic worth exploring. Let's take a look together!   Basic characteristics of luminol solution Luminol solution is a chemiluminescent reagent that produces strong fluorescence when reacting with certain chemicals, such as iron ions in hemoglobin. This characteristic makes luminol widely used in fields such as bloodstain detection and criminal investigation in forensic science. However, the stability of luminol solution is influenced by various factors such as temperature, light, humidity, etc. Shelf life at room temperature At room temperature, the shelf life of luminol solution is relatively short. This is because the luminol molecule itself is sensitive to light, heat, and oxidation, and these environmental factors will accelerate its decomposition, thereby reducing its chemiluminescence efficiency and detection sensitivity. Specifically, untreated luminol solution at room temperature may begin to degrade in a short period of time, affecting its effectiveness. It depends on factors such as humidity and light intensity in the storage environment, as well as the presence of potentially harmful pollutants to the reagents. Therefore, for applications with high requirements, it is not recommended to store luminol solution at room temperature for a long time However, it should be noted that even within the shelf life, the performance of luminol solution may be affected to some extent. Therefore, before conducting experiments or testing, it is recommended to inspect and control the solution to ensure that it still has good luminescent properties.   How to properly store luminol solution To ensure the longest possible shelf life of luminol solution at room temperature and maintain good performance, the following are some suggestions: 1. Storage environment: Store the luminol solution in a cool, dry, well ventilated place, away from direct sunlight and high temperatures. At the same time, stay away from harmful substances such as fire sources and oxidants. 2. Avoid vibration and shaking: During storage and transportation, severe vibration and shaking should be avoided to prevent chemical substances in the solution from reacting or precipitating. Shockproof packaging or cushioning materials can be used to protect the solution from vibration. 3. Container selection: Choose high-quality glass or plastic bottles as storage containers and ensure good sealing to prevent solution evaporation and contamination. At room temperature, the storage time of luminol solution is not long, but sealed storage under refrigeration can extend the storage time to less than one week. Although there is a retention period, to ensure the accuracy of experimental results, it is usually recommended to use it immediately or use it up within a short period of time. If the time is long or the solution changes, a new luminol solution needs to be prepared before each experiment to avoid affecting the experiment. By selecting appropriate storage environments, containers, and labeling methods, the shelf life of luminol solution can be extended while maintaining its good performance. As a manufacturer of luminescent reagents, Desheng has a complete range of luminol types, high sensitivity, and professional personnel to track and guide, helping to solve technical problems. If you have any relevant intentions, please click on the website to inquire about details!

In cell biology and biomedical research, cell culture medium is the foundation for maintaining cell growth, proliferation, and metabolism. Among them, HEPES buffer (4- (2-hydroxyethyl) -1-piperazinoethanesulfonic acid) plays a crucial role as an important additive in cell culture media. This article will explore in detail the application and importance of HEPES in cell culture media. Basic characteristics of HEPES HEPES is a non-ionic amphoteric compound with excellent buffering capacity. Its pH value is between 7.2-7.8 and it has no toxic effect on cells. These characteristics of HEPES make it an ideal choice for cell culture media, effectively maintaining the stability of the cell culture environment.   The role of HEPES in cell culture medium 1. Maintain pH stability Cells are highly sensitive to changes in pH, and even small fluctuations in pH can affect cell growth and metabolism. During the process of cell culture, the pH value of the culture medium will change due to the acidic and alkaline substances produced by cell metabolism. HEPES, as an excellent buffering agent, can quickly neutralize these substances and maintain the pH value of the culture medium within a relatively stable range. This stability is crucial for the growth and proliferation of cells, helping to maintain their normal physiological functions and metabolic status. 2. Provide osmotic pressure balance Maintaining appropriate osmotic pressure is also crucial during cell culture. Excessive or insufficient osmotic pressure can lead to an imbalance of intracellular and extracellular fluids, affecting cell growth and metabolism. HEPES, as an osmotic pressure regulator, can effectively regulate the osmotic pressure of the culture medium to maintain consistency with the osmotic pressure inside the cells. This helps maintain the normal morphology and function of cells, preventing damage to the cells. 3. Antioxidant effect During the process of cell culture, cells may be affected by oxidative stress, leading to cell damage and apoptosis. HEPES has a certain antioxidant effect, which can eliminate free radicals and other oxidative substances in the culture medium, and alleviate the damage of oxidative stress to cells. This antioxidant effect helps to protect cells from oxidative damage, improve cell survival rate and growth ability.   4. Improve the environment for cell growth In addition to the aforementioned effects, HEPES can also improve the cell growth environment. For example, it can increase the solubility and stability of the culture medium, making it easier for cells to absorb the required nutrients. Meanwhile, HEPES can also promote signal transmission and interaction between cells, helping to maintain normal communication and cooperation between cells. These effects all contribute to improving the growth rate and quality of cells.   Application examples of HEPES In biomedical research, HEPES is widely used in various types of cell culture media, including mammalian cells, insect cells, bacteria, etc. For example, in mammalian cell culture, commonly used DMEM and RPMI media are supplemented with HEPES as a buffer. These culture media can maintain stable pH and osmotic pressure, providing a favorable growth environment for cells. In summary, HEPES plays multiple important roles in cell culture media. It can maintain the pH and osmotic pressure stability of the culture medium, has antioxidant effects, and improves the cell growth environment. These effects all contribute to improving the growth rate and quality of cells, providing strong support for biomedical research. Therefore, it is best to choose a culture medium containing HEPES during cell culture. As a manufacturer of HEPES and other biological buffering agents, Desheng can supply raw material powders, which are easy to prepare and use, and offer batch quotations at flexible prices, making it a preferred partner for many enterprises. At present, there is a low price promotion. Interested parties are welcome to click on the website to inquire about details and purchase!

In the field of modern medicine, time is life, especially in the clinical laboratory stage, obtaining test results quickly and accurately is crucial for early diagnosis and treatment of illness. As a fundamental and frequently performed item in clinical diagnosis, the efficiency of blood testing directly affects the quality and speed of medical services. In response to this challenge, blood collection vessel coagulant technology has emerged, which significantly improves the efficiency of blood sample processing by accelerating the blood coagulation process, becoming one of the innovative technologies to enhance detection efficiency. The principle and application of coagulant technology Blood collection vessel coagulant, as the name suggests, is a chemical substance pre placed in the blood collection vessel. Its main function is to immediately activate the coagulation mechanism after blood sample collection, promoting rapid blood clotting. This process typically involves activating clotting factors in the blood, accelerating the conversion of fibrinogen into insoluble fibrin, and forming clots.   Improve detection efficiency 1. Shortening sample processing time: The addition of coagulants directly shortens the transition time of blood from liquid to solid state, from the conventional 60 minutes or more to a few minutes, improving the speed of sample processing. This means that doctors and laboratory technicians can start the subsequent testing steps in a shorter amount of time, accelerating the diagnostic process. 2. Ensuring sample quality: Through rapid coagulation, coagulants effectively reduce the possibility of hemolysis, cell lysis, and other issues during the processing of blood samples, thereby ensuring the biological stability of the samples and improving the accuracy of detection results. This is particularly important for the detection of time sensitive biomarkers, such as the determination of certain enzyme activities. 3. Simplified operation process: The use of coagulation inducing blood collection vessels simplifies the processing steps after blood collection, without the need for additional clotting agents or complex pre-processing, reducing the risk of operational errors and improving laboratory efficiency. Influencing factors and optimization strategies Although the application of coagulants has brought many conveniences, their effectiveness is influenced by various factors, including the type and concentration of coagulants, the volume of blood samples, and the degree of mixing after blood collection. The operator must ensure that the mixed blood collection tube is gently inverted 4-5 times after blood collection to ensure even distribution and full effectiveness of the coagulant. In addition, differences in temperature and blood composition may also affect coagulation speed, so adjustments to blood collection and processing procedures may be necessary under specific conditions.   Continuous innovation in technology With the advancement of technology, blood testing technology is also constantly evolving. In addition to the application of coagulants, emerging methods such as dry blood spot technology and laser blood testing technology are gradually changing the landscape of blood testing. The dry blood spot technology collects trace amounts of blood and places them on test strips, which not only reduces the invasiveness to the test subject, but also simplifies the sample collection and transportation process. Laser blood testing technology, with its non-invasive, fast, and high-precision characteristics, provides a new possibility for future blood testing. In summary, the blood collection vessel coagulant technology has opened up a new path to improve the efficiency of clinical blood testing by accelerating blood coagulation. It not only simplifies the pre-processing process of testing, but also helps to improve the accuracy and timeliness of testing, making it an indispensable part of modern medical laboratories. With the continuous advancement and innovation of related technologies, future blood testing will provide more solid support for the treatment of illness. In this fast-paced medical environment, every technological innovation that can accelerate the diagnosis and treatment process is a valuable contribution to human health and well-being. Hubei Xindesheng is a professional manufacturer of blood coagulants, with an independent research and development team and strict quality control by professional personnel. If you have purchasing needs, please feel free to contact us for purchase!

As an important application of modern biological science, paternity testing provides a scientific basis for resolving disputes over blood relationships. This process relies on DNA analysis, and sample collection is an important link in the entire identification chain. This article will focus on the commonly used sample type in paternity testing - blood, and how to use purple EDTA tubes for blood sample collection. 1、 Principles of paternity testing The core of paternity testing is to compare the DNA sequences of individuals to determine their biological relationships. Everyone's DNA sequence is unique, except for genetic information from parents. Therefore, by comparing the DNA of suspected fathers, mothers, and children, scientists can calculate the degree of genetic information matching between the two and determine whether there is a parent-child relationship. 2、 The role of purple EDTA tube Among various sample collection methods, blood is the preferred choice due to its high DNA content and ease of collection. However, blood collection naturally coagulates, which poses obstacles to DNA extraction. To solve this problem, scientists have invented blood collection tubes containing anticoagulants, among which the purple EDTA tube is specifically designed for this purpose. EDTA， Ethylene diamine tetraacetic acid (EDTA) is a calcium ion chelating agent that can prevent blood clotting and maintain sample integrity. The K2 EDTA powder in the purple EDTA tube is evenly distributed on the tube wall. When blood is collected into the tube, EDTA quickly binds to calcium ions in the blood, preventing the formation of blood clots and creating conditions for subsequent DNA extraction.   3、 Collection process The correct collection of blood samples is crucial for the accuracy of paternity testing. Collectors should ensure that all instruments are in a sterile state, clean the blood collection site with alcohol swabs, and reduce the risk of infection. Subsequently, using specialized needles and purple EDTA tubes, blood was drawn from the subject's elbow vein or other suitable location. After the collection is completed, the blood collection tube should be immediately reversed several times to fully mix the blood with EDTA and ensure anticoagulant effect. In addition, to maintain sample activity, exposure to high temperatures should be avoided, especially during the hot summer months. Samples should be transported under low temperature conditions, such as using specific containers with ice packs. 4、 Sample storage and transportation Proper storage of samples is equally important. The collected blood samples should be stored separately to avoid confusion with other samples. At the same time, the identity information of the subjects, including name, sample type, and collection date, should be clearly marked on the blood collection tube to ensure the accuracy of subsequent analysis. During transportation, the sample should be kept at a low temperature to prevent DNA degradation. Using insulated containers with ice cubes for transportation can maintain the stability of the sample until it is received and further processed in the laboratory. The application of purple EDTA tubes in paternity testing reflects the combination of scientific rigor and technological progress. By using these specially designed blood collection tubes, not only has the blood sample collection process been simplified, but the quality of the samples and the accuracy of the identification results have also been greatly improved. In the future, with the continuous advancement of technology, the technology of paternity testing will become more mature, providing society with more fair and accurate proof of blood relationship. By delving into the application of purple EDTA tubes in paternity testing, we not only see the important role of science and technology in modern society, but also recognize the responsibility and mission behind every scientific tool. In every detail of paternity testing, there is a respect for the dignity of life and a persistent pursuit of truth. Choosing Desheng as your supplier of ethylenediaminetetraacetic acid dipotassium and tripotassium salts will provide you with high-quality products, professional services, and reliable guarantees. If you have relevant purchasing intentions, please click on the website to inquire about details!

In the fields of fine chemicals and pharmaceuticals, Carbomer, as an important polymer, is highly favored due to its properties and wide applications. Carbopol, a derivative of polyacrylic acid esters, forms a three-dimensional network structure through cross-linking reactions, which endows it with a series of excellent physical and chemical properties. However, these properties are not fixed, but exhibit different appearances as the molecular weight of carbomer changes. This article will explore the intricate relationship between molecular weight and the properties of Carbopol, revealing how they work together to weave the diverse application scenarios of Carbopol.   1、 Molecular Weight: A Command Rod for Carbopol Performance The molecular weight of Carbopol is a key parameter that determines its rheological and application properties. In short, molecular weight refers to the length of a single polymer chain. For Carbopol, a higher molecular weight means longer chain segments, which are directly related to the viscosity and thickening effect of the material. When the molecular weight of carbomer increases, the interactions between its molecular chains are enhanced, forming a denser network structure, thereby significantly increasing the viscosity of the solution or dispersion system. This high viscosity characteristic makes Carbopol an ideal thickener and stabilizer, shining in fields such as skincare, formulations, and industrial coatings. 2、 Rheology: Direct Mapping of Molecular Weight Rheology refers to the ability of a substance to flow and deform under external forces. For Carbopol, its rheological properties are closely related to its molecular weight. High molecular weight carbomers exhibit non Newtonian fluid properties, where their viscosity decreases with increasing shear rate, a phenomenon known as "shear thinning". In practical applications, this characteristic is very beneficial. For example, when applying skincare cream or ointment, the product can easily unfold, but once the applied pressure disappears, it can quickly return to its original state and maintain good morphological stability. 3、 Molecular Weight Distribution: Shaping Complex Properties In addition to the molecular weight itself, molecular weight distribution (MWD) is also an important factor affecting the performance of Carbopol. The molecular weight distribution reflects the proportion of different molecular weight fractions in a polymer. Carbopol with a wider molecular weight distribution contains more molecular chains of different lengths inside, which not only increases the complexity of the material, but also enhances its adaptability and functionality under specific conditions.   4、 Harmonious resonance between molecular weight and performance The relationship between molecular weight and carbomer performance is like a carefully choreographed dance, with every movement accurate and every turn full of meaning. By regulating the molecular weight of Carbopol, scientists and engineers can create products that meet different needs. In the future, with a deeper understanding of Carbopol and advances in technological innovation, we have reason to believe that Carbopol will demonstrate even more amazing potential and continue to play an important role in the fields of fine chemicals and pharmaceuticals.   Through the exploration in this article, it is not difficult to find that the relationship between the molecular weight of Carbopol and its properties is much richer and more complex than imagined. It is precisely this subtle interaction that endows Carbopol with many possibilities, making it an indispensable high-performance material in many fields. Hubei Xindesheng Material Technology Co., Ltd. specializes in producing various types of blood collection tube additive raw materials and Carbopol. After more than ten years of research and development, Carbopol has the characteristics of stable process, small batch differences, and obvious price advantages. If you have any purchasing needs, please feel free to visit the official website of Hubei Xindesheng Materials to view our product series, or contact us for consultation and ordering!

In the field of biochemistry, the determination of enzyme activity is a crucial task. Enzyme activity is an essential indicator for evaluating the metabolic status of organisms and diagnosing diseases. In recent years, MAOS reagent, as a novel chromogenic substrate, has shown excellent performance in enzyme activity assays, attracting widespread attention and research. This article will delve into the outstanding performance of MAOS in enzyme activity assays.   Characteristics and advantages of MAOS MAOS， The full name is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt monohydrate, which is a highly water-soluble derivative of aniline. As a chromogenic substrate, it has shown particular advantages in enzyme activity assays. Firstly, MAOS has a long ultraviolet absorption wavelength, which can reach up to 630 nanometers. This characteristic enables MAOS to reduce background interference during the measurement process, improving the sensitivity and accuracy of the measurement. Secondly, MAOS has a wide adaptability range to pH values and can maintain stable color development effects within a wide pH range, which enables MAOS to adapt to suitable pH conditions for different enzymes and thus more accurately measure enzyme activity. In addition, MAOS also has a high molar absorptivity, which can produce significant color changes and facilitate observation and recording of experimental results.   Application of MAOS in Enzyme Activity Measurement 1. High sensitivity: Due to its long UV absorption wavelength and high molar absorptivity, MAOS can produce significant color changes in enzyme activity assays, greatly increasing the sensitivity of the measurement. Even at lower enzyme concentrations, accurate and reliable measurement results can be obtained. 2. High accuracy: MAOS has a wide adaptability range to pH values and can maintain stable color development under suitable pH conditions for different enzymes. This enables MAOS to reduce errors caused by pH changes and improve the accuracy of enzyme activity assays. Meanwhile, MAOS can also reduce the influence of other potential interfering factors, such as ion strength, temperature, etc., further improving the accuracy of the measurement. 3. Easy to operate: MAOS, as a chromogenic substrate, is relatively easy to operate in enzyme activity assays. Simply mix MAOS with the enzyme solution to be tested and observe the color change for measurement. Compared with traditional enzyme activity determination methods, MAOS does not require complex instrument equipment, reduces experimental costs, and improves experimental efficiency.   Specific cases of MAOS in enzyme activity determination Taking uricase as an example, uricase is an important metabolic enzyme, and its activity is of great significance for evaluating renal function and metabolic status. The traditional methods for measuring uricase activity typically use biphenyl colorimetric reagents such as DAB, but these reagents may be carcinogenic or mutagenic, and the operation is complex and the sensitivity is low. Using MAOS as a chromogenic substrate for uricase activity determination can not only increase the sensitivity and accuracy of the measurement, but also reduce experimental costs and operational difficulties. In the experiment, simply mix MAOS with uricase solution and observe the color change to quickly and accurately determine the activity of uricase. MAOS, as a novel chromogenic substrate, has demonstrated excellent performance in enzyme activity assays. The high sensitivity, accuracy, and ease of operation of MAOS make it widely applicable in the field of biochemistry. With the continuous development of technology in the future, MAOS is expected to be applied in more types of enzyme activity assays, providing more accurate and reliable experimental data support for the evaluation of biological metabolic status and disease diagnosis. Desheng is a manufacturer of the new Trinder's reagents and can provide a range of reagents such as MAOS. Independently developed and produced, with small inter batch differences and sensitive response, it has important value in clinical diagnosis. If you have any purchasing needs, please feel free to contact us for consultation and ordering!

Luminol solution has attracted much attention for its applications in the field of chemiluminescence, especially in forensic science, biochemistry, and environmental monitoring. This compound can emit a bright blue white light under appropriate conditions, making invisible bloodstains clear and visible, thus becoming an indispensable tool in crime scene investigation. However, the stability of luminol solution directly affects its reliability and effectiveness in practical applications. This article will delve into the stability issues of luminol solution, including its influencing factors, storage conditions, and strategies to improve stability. Basic characteristics of luminol solution Luminol, also known as 3-amino-phthalic anhydride (C8H7N3O2), is relatively stable at room temperature. It belongs to artificially synthesized organic compounds, and the amino and hydrazide groups contained in its molecular structure endow it with the ability to emit light under specific conditions. When luminol solution is exposed to an alkaline environment in the presence of hydrogen peroxide and appropriate catalysts (such as iron ions), an oxidation reaction occurs, producing high-energy intermediates, which then release energy in the form of light, forming the chemiluminescence phenomenon that people see. Factors affecting the stability of luminol solution 1. Light and oxygen Light is one of the main factors causing instability of luminol solution. Light energy induces photolysis of luminol molecules in solution, thereby reducing their luminescence efficiency. Similarly, the presence of oxygen accelerates the oxidation process of luminol and reduces its lifespan. Therefore, avoiding light and isolating air are basic requirements for maintaining the stability of luminol solution. 2. Temperature Temperature also has a significant impact on the stability of luminol solution. Under high temperature conditions, the degradation rate of luminol accelerates, which not only shortens the lifetime of the solution, but may also affect the intensity and duration of luminescence. Therefore, low-temperature storage is a useful means to maintain the stability of luminol solution. 3. PH value Appropriate pH conditions are a prerequisite for the smooth progress of the luminescent reaction of luminol. Generally speaking, alkaline environments (such as pH 10-11) are favorable for the chemiluminescence reaction of luminol. However, the pH value (either too alkaline or too acidic) can disrupt the molecular structure of luminol, affecting its stability and luminescent properties.   Preservation strategy of luminol solution 1. Ready to use, ready to use Given the instability of luminol solution, a suitable strategy is to prepare it immediately before experimentation or analysis to avoid performance degradation caused by prolonged storage. 2. Refrigerated storage If it is necessary to prepare in advance, the luminol solution should be refrigerated (about 4 ℃) and stored in a sealed container away from light, which can significantly extend its shelf life to about a week. 3. Add stabilizer Adding an appropriate amount of antioxidants or stabilizers, such as ascorbic acid or EDTA, to the solution can slow down the oxidation process of luminol and improve its stability. Precautions for laboratory operations When preparing and using luminol solution in the laboratory, the following points should be noted to maintain its performance: 1. Use high-purity chemical reagents to avoid additional unstable factors introduced by impurities. 2. Control the temperature and pH value during the solution preparation process to ensure suitable conditions 3. Reduce the contact time between the solution and air, operate quickly and under an inert gas atmosphere (such as using nitrogen protection). 4. After the experiment is completed, promptly clean the residual solution to avoid contamination and deterioration caused by long-term storage. The stability of luminol solution is directly related to its effectiveness and reliability in various fields of application. By deeply understanding the key factors that affect its stability and adopting corresponding preservation measures and operating techniques, the service life of luminol solution can be extended, ensuring the accuracy and repeatability of experimental results. With the deepening of research on luminol and its luminescence mechanism, it is expected that more stable and sensitive luminol derivatives or improved preservation techniques will be developed in the future, further expanding its application scope. As a manufacturer of luminescent reagents, Desheng has a complete range of luminol types, high sensitivity, and professional personnel to track and guide, helping to solve technical problems. If you have any relevant intentions, please click on the website to inquire about details!

In modern clinical diagnosis and disease screening, procalcitonin (PCT) testing is widely used as an important biomarker to assess the severity of infection and predict disease prognosis. In this testing process, sodium citrate anticoagulant tubes have become an indispensable tool due to their anticoagulant properties. This article will explore the characteristics of sodium citrate anticoagulant test tubes, the importance of procalcitonin detection, and the advantages of combining the two.   Characteristics of Sodium Citrate Anticoagulant Test Tube Sodium citrate anticoagulant test tube is a specialized test tube used for blood sample collection and preservation, with its core advantage being its anticoagulant properties. Sodium citrate can bind with calcium ions in the blood to form insoluble calcium citrate, thereby inhibiting the blood clotting process and maintaining the liquid and stable state of the blood sample. This characteristic makes sodium citrate anticoagulant test tubes widely used in various clinical tests. The importance of procalcitonin detection Procalcitonin is a protein secreted by thyroid C cells, and under normal circumstances, its content in serum is extremely low. However, in cases of bacterial infection, especially systemic infection, the synthesis of procalcitonin significantly increases and increases with the severity of the infection. Therefore, procalcitonin testing has become an important means of assessing the severity of infection, distinguishing infection from viral infection, guiding antibiotic use, and predicting disease prognosis.   Application of sodium citrate anticoagulant test tube in procalcitonin detection 1. Ensuring sample quality: In procalcitonin testing, the integrity and stability of blood samples are crucial for the accuracy of the results. Sodium citrate anticoagulant test tubes, with their excellent anticoagulant properties, effectively prevent blood clotting, ensure the stability of samples during collection, transportation, and testing, thereby improving the reliability of test results. 2. Easy to operate: using sodium citrate anticoagulant tubes for venous blood collection, the operation is simple and fast, reducing errors and contamination risks during the operation process. At the same time, the design of the test tube also facilitates subsequent detection and processing, improving work efficiency. 3. Wide applicability: Procalcitonin testing has a wide range of clinical applications, including emergency, ICU, surgery and other departments. The sodium citrate anticoagulant test tube, as the standard blood collection tool for this test, can meet the needs of different departments and testing requirements, and has good universality and flexibility. 4. Assisted clinical decision-making: By combining procalcitonin testing with sodium citrate anticoagulant test tubes, doctors can more accurately assess the patient's infection status, degree of infection, and treatment effectiveness. This not only helps to adjust the diagnosis and treatment plan in a timely manner, but also helps to reduce medical costs and improve patient prognosis.   Application examples and precautions In practical applications, procalcitonin detection is usually performed using methods such as immunofluorescence assay or colloidal gold assay. When collecting blood, it is necessary to strictly follow the operating procedures and use sodium citrate anticoagulant test tubes for intravenous blood collection, and immediately send them for testing to ensure the freshness and timeliness of the sample. At the same time, when interpreting the results of procalcitonin testing, doctors should comprehensively analyze the patient's clinical manifestations, other laboratory tests, and imaging results to improve the accuracy and reliability of diagnosis. In summary, sodium citrate anticoagulant tubes play an important role in procalcitonin detection. Its excellent anticoagulant performance ensures the integrity and stability of blood samples, improving the accuracy and reliability of test results. Meanwhile, procalcitonin testing, as an important means of assessing the severity of infection and predicting disease prognosis, has broad clinical application prospects. In the future, with the continuous advancement of detection technology and the deepening of clinical applications, the combination of sodium citrate anticoagulant tubes and procalcitonin detection will provide more powerful support for clinical diagnosis and treatment. Sodium citrate is one of the main products of Hubei Xindesheng Company, which stands out in the market for its high purity, good water solubility, and excellent efficacy, and is highly praised by domestic and foreign customers. Complete variety, welcome to purchase!

The red blood cell osmotic fragility test is an important experiment in the field of hematology, aimed at evaluating the resistance of red blood cells to different concentrations of hypotonic solutions, in order to understand the characteristics and functional status of red blood cell membranes. This experiment is of great significance for diagnosing various diseases such as anemia, jaundice, and liver disorders. In the experiment of red blood cell osmotic fragility, the selection of anticoagulant is crucial, and heparin lithium tubes are widely used due to their advantages.   Application advantages of heparin lithium tube 1. Excellent anticoagulant effect Heparin lithium, as a commonly used anticoagulant, has excellent anticoagulant effects. It interferes with the interaction between thrombin and fibrinogen, preventing the formation of fibrin and thus preventing blood clotting. In the red blood cell osmotic fragility experiment, the rapid anticoagulant effect of heparin lithium can ensure the stability of blood samples during collection, transportation, and storage, avoiding the influence of blood coagulation on the experimental results. 2. Low impact on red blood cells Compared to other anticoagulants, heparin lithium has a smaller impact on red blood cells. It does not change the volume of red blood cells, nor does it cause hemolysis. This enables the use of heparin lithium anticoagulated blood samples in red blood cell osmotic fragility experiments to more accurately reflect the true performance of red blood cells in hypotonic solutions, thereby improving the accuracy of experimental results. 3. Easy to operate The heparin lithium anticoagulant tube has been pre loaded with an appropriate amount of anticoagulant during the production process, and can be directly used for blood sample collection without additional operation. This ready to use design not only simplifies the experimental steps, but also improves work efficiency. Meanwhile, heparin lithium anticoagulant tubes have good stability and compatibility, making them suitable for various experimental scenarios. 4. Wide applicability Heparin lithium anticoagulant is not only suitable for red blood cell osmotic fragility testing, but also widely used in biochemical testing, routine biochemical testing, and liver disease examination of patients after hemodialysis. Its wide range of applications proves its reliability and timeliness in practical applications.   Practical considerations 1. Precautions during blood collection process When using heparin lithium anticoagulant tubes for red blood cell osmotic fragility experiments, attention should be paid to some details during the blood collection process. For example, to ensure that the blood collection tube is clean and sterile, and to avoid contamination; When collecting blood, avoid forcefully squeezing or shaking the test tube to prevent damage to red blood cells; After blood collection, it is necessary to mix the blood and anticoagulant in a timely manner to ensure the anticoagulant effect. 2. Control of experimental conditions The red blood cell osmotic fragility experiment requires high experimental conditions and strict control of parameters such as experimental temperature and solution concentration. During the experiment, it is necessary to ensure that the temperature of all test tubes and solutions is consistent to avoid the influence of temperature differences on the experimental results. At the same time, it is necessary to accurately prepare low-permeability solutions of different concentrations to ensure the accuracy of experimental results. 3. Interpretation and analysis of results When interpreting experimental results, it is important to observe the behavior of red blood cells in different concentrations of hypotonic solutions. By recording the concentration of the solution at which red blood cells begin to hemolysis and undergo complete hemolysis, the minimum and maximum resistance of red blood cells can be calculated, thereby evaluating their osmotic fragility. At the same time, comprehensive analysis should be conducted in conjunction with other laboratory test results to obtain accurate diagnostic conclusions. In summary, heparin lithium tubes have excellent application advantages in red blood cell osmotic fragility experiments. Its excellent anticoagulant effect, minimal impact on red blood cells, easy operation, and wide applicability make it an ideal anticoagulant for red blood cell osmotic fragility experiments. However, in practical applications, attention still needs to be paid to the details of the blood collection process, control of experimental conditions, and interpretation and analysis of results. Through scientific and rational operation and rigorous experimental design, the advantages of heparin lithium tubes in red blood cell osmotic fragility experiments can be fully utilized, improving the accuracy and reliability of experimental results. Hubei Xindesheng is a professional manufacturer of heparin lithium and other blood collection tube additives, with an independent research and development team and strict quality control by professional personnel. If you have purchasing needs, please feel free to contact us for purchase!

The luminol reaction, with its chemiluminescence properties, has become an indispensable detection method in forensic science, biochemistry, and scientific research fields. This reaction can reveal trace bloodstains with good sensitivity, detect oxidation reactions, or be used for quantitative analysis of biomarkers. In order to ensure the effectiveness of the luminol reaction, strict environmental conditions are required for its implementation, including chemical composition, physical parameters, and operational steps. This article will delve into the specific environmental requirements of the luminol reaction, in order to provide guidance for related experimental operations.   Chemical composition and concentration 1. Luminol: As the core substance of the reaction, the purity and concentration of luminol directly affect the luminescence intensity. Generally speaking, luminol is stored in powder form and needs to be accurately weighed and dissolved in a suitable solvent before use. The concentration control within certain ranges, either too high or too low, may affect the luminous efficiency. 2. Oxidizing agent: The most common oxidizing agent is hydrogen peroxide (H ₂ O ₂), with a concentration of typically 30%, as this concentration can provide sufficient oxidizing ability without excessively oxidizing luminol, resulting in rapid decay of luminescence. The purity of the oxidant is equally important, as any impurities may become additional sources of luminescence or inhibit the reaction. 3. Catalyst: Iron ions (commonly found in hemoglobin) are common catalysts in the natural environment, but in the laboratory, other transition metal ions such as potassium ferrocyanide may also be used as substitutes. The type and concentration of catalyst directly affect the reaction rate and luminescence intensity, and need to be carefully adjusted according to the experimental purpose. 4. Alkaline medium: Concentrated ammonia water is a commonly used alkaline reagent that provides an alkaline environment for the luminol reaction. The concentration and volume of ammonia water need to be carefully controlled to ensure that luminol is fully dissolved and converted into a luminescent form.   Physical parameters 1. Temperature: The efficiency of the luminol reaction is significantly affected by temperature, and generally, room temperature (about 20-25 ° C) is the suitable reaction temperature. Low temperature can slow down the reaction rate, while high temperature may lead to degradation of luminol, affecting the luminescence effect. 2. Light: Luminol reaction and its products are sensitive to light, so the experiment should be conducted in a dark room or under shading conditions to avoid background light interference with luminescence detection. 3. PH value: The ideal pH range for the luminol reaction is usually alkaline conditions. Even small changes in pH can affect luminescence efficiency, so it is necessary to use pH test strips or electrodes to monitor and adjust the pH of the solution. Operation steps and precautions 1. Order and Mixing: The order and mixing method of adding reactants are also crucial. It is generally recommended to dissolve luminol in an alkaline solution first, and then slowly add an oxidant to avoid intense reactions that may cause local overheating or rapid disappearance of luminescence. 2. Avoid pollution: During the experimental process, efforts should be made to minimize the contamination of foreign substances, especially microorganisms, dust, or oil, which may consume oxidants or catalyze non target reactions, interfering with the luminescence effect. 3. Safety measures: Due to the strong oxidizing agent involved in the luminol reaction, appropriate personal protective equipment such as gloves and goggles must be worn during operation, and it should be carried out in a well ventilated environment to prevent chemical splashing or inhalation of harmful gases. The successful implementation of the luminol reaction relies on a series of finely regulated environmental conditions. From the proportioning of chemical components, control of physical parameters to the mastery of operational details, every step needs to be executed rigorously. By understanding and following these requirements, researchers and forensic experts can fully utilize the potential of the luminol reaction to achieve sensitive detection of weak biomarkers or exposure of micro traces at crime scenes. In practical applications, continuous optimization of reaction conditions, combined with modern technologies such as fluorescence imaging systems, will further push the scientific application boundaries of the luminol reaction. As a manufacturer of luminescent reagents, Desheng has a complete range of luminol types, high sensitivity, and professional personnel to track and guide, helping to solve technical problems. If you have any relevant intentions, please click on the website to inquire about details!