Biological Buffer TRIS (CAS77-86-1) CAS.77-86-1 Color White

Introduction and application of biological buffer TRIS (CAS77-86-1) CAS.77-86-1 color white

Keywords: Biological buffer, TRIS, CAS77-86-1, Tris

The full name of Tris is tris(hydroxymethyl)aminomethane, another name is tromethamine, and its CAS number is 77-86-1. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.

Tris is widely used in acute metabolic and respiratory acidemia. It is an alkaline buffer, which has a good buffering effect on metabolic acidosis and enzyme activity, and can cause hypoglycemia, hypotension, and nausea. , Vomiting, can also inhibit breathing, or even stop breathing. Excessive intake or renal insufficiency can cause alkalemia. When used as a biological buffer and acid titration standard substance, the purity requirements are relatively high, generally 99.9%-100.1%.

Tris (Tris(hydroxymethylaminomethane)) is often used as a biological buffer, and is often formulated with pH values ​​of 6.8, 7.4, 8.0, and 8.8. Its structural formula and pH value vary greatly with temperature. Generally, the PH value decreases by 0.03 every time the temperature rises by one degree. Tris (Trishydroxymethylaminomethane) is widely used in the preparation of buffers in biochemistry and molecular biology experiments. The TAE and TBE buffers commonly used in biochemical experiments (for the dissolution of nucleic acids) require Tris. Because it contains amino groups, it can undergo condensation reactions with aldehydes.

Tris (Tris(hydroxymethyl)aminomethane) is a weak base, and its pKa is 8.1 at room temperature 25°C. According to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is about 10.5. Generally, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of the pH value. But at the same time, attention should be paid to the influence of temperature on the pKa of Tris. Since the Tris buffer is a weakly alkaline solution, DNA in such a solution will be deprotonated, thereby improving its solubility. People often add EDTA to Tris hydrochloric acid buffer to make "TE buffer". TE buffer is used for DNA stabilization and storage. If you change the pH-adjusting acid solution to acetic acid, you get "TAE buffer" (Tris/Acetate/EDTA), and change to boric acid to get "TBE buffer" (Tris/Borate/EDTA). These two buffers are commonly used in nucleic acid electrophoresis experiments. TAE and TBE prepared by Tris are the most commonly used reagents for DNA electrophoresis, and TE (pH 8.0) is mainly used to dissolve DNA. (TE is the collective name of Tris plus EDTA.) 1MTris-HCl6.8 and 1.5MTris-HCl8.8 are the most commonly used reagents for SDS-PAGE.

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