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|Brand:||Desheng||Apperance:||White Crystal Powxer|
|Molecular Weight:||221.32||Molecular Formular:||C9H19NO3S|
1135-40-6 DNA RNA Extraction Kit,
Buffer DNA RNA Extraction Kit,
1135-40-6 nucleic acid extraction kit
DNA/RNA extraction kit buffer N-Cyclohexyl-3-aminopropanesulfonic acid/CAPS 1135-40-6
|Full Name||N-Cyclohexyl-3-aminopropanesulfonic acid||Molecular weight||221.32|
|Appearance||White crystal powder||Purity||≥99%|
CAPS reagent, cyclohexylamine propane sulfonic acid, is a sulfamate with amphiphilic nature. It is most widely used in water-based polyisocyanate coatings and biological buffers for biochemical experiments such as HPLC separation and SDS-PAGE gel electrophoresis. .
When used as a biological buffer, CAPS is similar to Tris and MOPS. Both can be used for running buffers, but there are differences. We can first understand the requirements of running buffers. Electrophoresis refers to the movement of charged particles under the action of an electric field, and the movement rate (mobility) of the charged particles is fixed under certain conditions. In biochemical experiments, the charged particles are usually protein micelles, nucleic acids, etc., and different proteins or nucleic acids have different migration rates and will separate from each other. Since protein and nucleic acid particles are very sensitive to pH, buffers must be added to the electrophoresis solution to protect the protein or nucleic acid.
Cyclohexylamine propane sulfonic acid CAPS
When the voltage of the applied electric field is fixed and the conditions of the electrophoresis time are the same, the mobility of different electrophoretic particles is different. Therefore, the size or type of protein or nucleic acid is different, and the mobility is different. The difference in mobility of different particles, that is, the separation distance, is proportional to the voltage and the electrophoresis time. Depending on the size of the electrophoresis particles, the electrophoresis buffer used is also different.
CAPS buffer: suitable for electrophoresis of high molecular weight proteins (greater than 20KD), that is, western blot. Because the electrophoresis buffer made by CAPS only contains EDTA and methanol, protein gel electrophoresis PVDF is not only used for protein separation, but also for subsequent protein sequencing.
Tris buffer: usually used for electrophoresis of nucleic acid DNA, that is, Southern blot. Among them, TEA is used for DNA fragments larger than 13kb with good separation effect and can be used to recover DNA; TBA is used for DNA fragments less than 1kb, but the boric acid in it will affect DNA recovery and subsequent enzyme reaction experiments. Tris can also be used for low molecular weight protein blotting experiments, but glycine cannot be used for protein sequencing. Tris-Tricine+EDTA buffer system is required.
MOPS buffer: Usually used to prepare RNA running buffer during electrophoresis, that is, Northern blot.
In addition to the aforementioned CAPS, Tris, and MOPS, there are some other electrophoresis buffers. In the entire electro-blotting experiment, the cost of the buffer is less than that of nucleic acid dyes, proteins, etc. It is recommended to use high-quality Buffer raw materials ensure the experimental effect. Desheng specializes in the research and development and production of various biological buffers, which can meet the buffer requirements of different experiments.
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