CAS 1135-40-6 CAPS Buffer 3-(Cyclohexylamine)-1-Propanesulfonic Acid
How much do you know about the application and configuration of CAPS
The full name of CAPS is 3-cyclohexylaminopropanesulfonic acid. The appearance is white powder crystal particles, which can be dissolved in water but the dissolution rate is slow. The CAS number is 1135-40-6 and the molecular formula is C9H19NO3S. It is a biological buffer and is mostly used in biochemical diagnostic kits, PCR diagnostic kits, DNA extraction kits and RNA extraction kits. But what other applications besides these places? The following will introduce the applications of CPSA.
||White crystal powder
3-(cyclohexylamine)-1-propanesulfonic acid is abbreviated as CAPS, which is a biological buffer. CAS1135-40-6, its molecular formula is C9H19NO3S, molecular weight is 221.32, it is white crystalline powder.
CAPS white crystalline powder
Buffer capacity of 3-(cyclohexylamine)-1-propanesulfonic acid buffer solution:
Adding a small amount of strong acid or alkali to the buffer solution will not change the pH value of the solution much, but if the amount of acid and alkali is large, the buffer solution will lose its buffering effect. This shows that its buffer capacity is limited.
The buffer capacity of the buffer solution is related to the concentration of the components that make up the buffer solution. The buffer solution composed of 0.1mol·L-1HAc and 0.1mol·L-1NaAc has a greater buffering capacity than the buffer solutions of 0.01mol·L-1HAc and 0.01mol·L-1NaAc. This point can be confirmed by calculation. But the concentration of the components of the buffer solution should not be too high, otherwise, the effect between ions cannot be ignored.
When the ratio of the two components of the buffer solution is not 1:1, the buffering effect is reduced and the buffering capacity is reduced. When c (salt)/c (acid) is 1:1, the △pH is the smallest and the buffering capacity is large. Whether for acid or alkali, it has a greater buffering effect. The pH value of the buffer solution can be calculated by the following formula:
At this time, the buffer capacity is large. The farther the ratio of the buffering components is from 1:1, the smaller the buffering capacity, and it may not even play a buffering role. For any buffer system, there is an effective buffer range, which is roughly within one pH unit on each side of pKaφ (or pKbφ).
Weak acid and its salt (weak acid and its conjugate base) system pH=pKaφ±1
Weak base and its salt (weak base and its conjugate acid) system pOH=pKbφ±1
For example, the pKaφ of HAc is 4.76, so HAc and NaAc are suitable for preparing a buffer solution with a pH of 3.76 to 5.76, which has a greater buffering effect in this range. The buffering capacity is the largest when preparing a buffer solution with pH=4.76, at this time (c(HAc)/c(NaAc)=1. 3-(cyclohexylamine)-1 propanesulfonic acid has a wide range of uses, 3-cyclohexylaminopropanesulfone Acid (CAPS) is relatively stable in nature, with a pKa value of 10.4 at 25°C and a pH buffer range of 9.7-11.1. It is widely used in biochemical analysis and in vitro diagnostic industries.
CAPS 3-(cyclohexylamine)-1 propanesulfonic acid has a wide range of uses:
CAPS is widely used as a biological buffer: used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits; used in enzymatic chemistry and HPLC separation of alkaline drugs buffer. When CAPS is used as a biological buffer, it needs better purity. Generally, it needs to be analytically pure. When used in industry, the purity requirements are relatively low, but different treatments must be made for different
CAPS is used in industry: for new coatings and new materials, CAPS is also a raw material for manufacturing welding materials, air-conditioning equipment and manufacturing metal lithium.
Used as analytical reagent, heat exchange carrier, and also used in the pharmaceutical industry. Used for air conditioning, but also for making fireworks; dry batteries and making metal lithium, and also used as flux and desiccant.
For the coating industry, it is used as a water-based isohydrogen ester curing agent. The water-based isocyanate curing agent can coexist stably with resins containing active groups (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time at room temperature.
Desheng Technology has been developing and producing in vitro diagnostic reagents for blood testing since 2005, and has in-depth research on the new Trinder's reagents, biological buffers and chemiluminescence reagents for chromogen substrates.
1. CAPS is used as a biological buffer: CAPS is mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as a buffer for enzymatic chemistry and HPLC separation of alkaline drugs. CAPS can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at a pH of 10.5. It can also be dissolved in deionized water and adjusted to pH 11.0 for purification of fibronectin.
CAPS needs better purity when used as a biological buffer, and generally requires analytical purity. When used in industry, the purity requirements are relatively low, but different treatments must be made for different applications.
2. CAPS is used in industry: used for new coatings and new materials. CAPS is also the raw material for manufacturing welding materials, air conditioning equipment and manufacturing metal lithium.
3. Used as analytical reagent, heat exchange carrier, and also in the pharmaceutical industry. For air conditioning, it is also used to make fireworks, dry batteries and metal lithium, and also used as flux and desiccant.
4. For the coating industry, it is used as a water-based isocyanate curing agent. The water-based isocyanate curing agent can coexist stably with resins containing active groups (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time at room temperature.
The CAPS application is roughly that, the following will introduce the conventional configuration method of CAPS
Since the CAPS buffer is mainly composed of CAPS and deionized water, adjust the pH value with sodium hydroxide to avoid contact with air. Add deionized water to CAPS to 900ml, adjust the pH to 11.0 with 2mol/L NaOH, then dilute to 1L and store at 4ºC. Generally, a buffer with a high methanol concentration has a good effect on the transfer of low molecular weight proteins, while a buffer with a low methanol concentration or even no methanol is conducive to the transfer of high molecular weight proteins. The concentration of methanol in the CAPS electro-imprinting buffer is 0-20%, so the CAPS electro-imprinting buffer is more suitable for the transfer of low molecular weight proteins.