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CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid

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CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid

CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid
CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid
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Large Image :  CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid

Product Details:
Place of Origin: China
Brand Name: De Sheng
Certification: ISO9001
Model Number: CAPS
Payment & Shipping Terms:
Minimum Order Quantity: 10g
Price: Negotiable
Packaging Details: plastic bottle/paper box
Delivery Time: 1-3 days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100kg/month
Detailed Product Description
Item: CAPS Apperance: White Crystal
Package: Plastic Bottle CAS#: 1132-61-2
MOQ: 10g Application: Biochemistry Buffer
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CAS 1135-40-6 Good Buffer Solutions

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CAPS Cyclohexylamine Propanesulfonic Acid

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99% Cyclohexylamine Propanesulfonic Acid

Application Of CAPS Cyclohexylamine Propanesulfonic Acid CAS1135-40-6 In WB Experiment

 

Brief introduction

Full Name N-Cyclohexyl-3-aminopropanesulfonic acid Molecular weight 221.32
Abbreviation CAPS Molecular formula C9H19NO3S
CAS No 1135-40-6 ENIECS 214-492-1
Appearance White crystal powder Purity ≥99%

 

Molecular Structure

CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid 0

Good's buffer, which is zwitterionic buffer. This type of buffer is generally a sulfamic acid-based organic compound. After the solution is formulated, the pH is relatively stable and does not react with various enzymes and chemical reagents. It is mainly used in biology and medicine.
 
Good’s buffer has the following general characteristics
(1) The solubility in water is high;
(2) acid and alkali dissociation constant (pKa value) is stable, generally in 6~8, the ability to release protons is stable, and little affected by the ion concentration, solution composition and temperature;
(3) has a low cell membrane permeability, it is not easy to penetrate the biofilm;
(4) can chelate with metal ions in low molar mass, but not generate precipitation with metal ions;
(5) Chemical stability is high;
(6) the light absorption in the ultraviolet and visible wavelength ranges is small;
(7) the possibility of salting out with the organic macromolecules in the organism is small;
(8) the high purity salt is easy to obtain; (9) does not participate in the biochemical reaction process, does not interfere with the biochemical reaction process.
At present, the commonly used Good's buffers are shown in the following table, in which the concentration of buffer and sodium hydroxide is 0.1mol/L.
 

Application of CAPS

At present, the PVD transmembrane of protein has not used Tris-gly, SDS, 20% methanol buffer, because there is glycine in the buffer, so it is difficult to distinguish whether glycine is brought in by Buffer or the protein itself when sequencing. Now the transmembrane buffer used for sequencing is 10 mM/1 CAPS 10% methanol, and the methanol concentration range in CAPS imprint buffer is 0-20%. In general, the buffer with high methanol concentration is paired. The transfer efficiency of low molecular weight proteins is good, while the buffer with low or no methanol concentration is beneficial to the transfer of high molecular weight proteins.

 

Advantage of CAPS

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

 

Preparation of CAPS

prepare 0.1mol/L buffer solution with the volumetric flask. During the configuration process, the buffer needs high temperature dehydration, and cooling in the dryer; preparation of 0.1mol/L sodium hydroxide solution. According to the formula in the above table, the corresponding pH buffer can be obtained, sodium hydroxide is mainly used to adjust the pH.

 

CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid 1

 

CAS 1135-40-6 Good Buffer Solutions CAPS Cyclohexylamine Propanesulfonic Acid 2

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