Cas No 29915-38-6 Good Buffer Biological buffer TAPS 3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid
Synthesis process of biological buffer TAPS
TAPS, its full name is N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, is a zwitterionic buffer, widely used in the fields of biochemistry and molecular biology. It is part of the Tris series of buffers, with a pH buffer range of 7.7-9.1, soluble in water (25g/50ml). TAPS, as a commonly used buffer system in DNA screening systems, can also be used as a buffer component of RNA samples. It is suitable for electron transfer and phosphorylation studies of chloroplast thin-layer preparations, and protects oxyhemoglobin from oxidation to high iron during freeze-drying. Hemoglobin can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
Chinese name N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid molecular weight 243.28
The chemical structure of TAPS
Preparation method of taps solution (about 1-2l)
(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml
(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml
As a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of thin-layer chloroplast preparation. It can protect oxyhemoglobin from oxidizing to methemoglobin during freeze-drying process, and can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.
Biological buffer for separation of dyes in chromatography
Chromatography is a laboratory technique, which is usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Due to the retention of ionizable compounds.The mobile phase pH is particularly sensitive, so a buffer should be added to the system to control this variable.
Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers include taps, which have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.
One of the most suitable biological buffers for cell culture
The most important characteristics of good's buffer.One is that they are not toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the experiment,The pH value of is under control. Due to their special functions, many good's Buffers / biological buffers.It is considered to be an ideal choice for cell separation, cell culture, enzyme analysis and many other biochemical applications.
1. Whether to choose powder or liquid
If you need liquid, you can prepare TAPS ready-to-use buffer according to the small amount of customer demand. The specifications are usually 200mL, 500mL, 1L, etc. Once the goods arrive, it is very convenient to use immediately, but the price is relatively high.
If the demand is large or needs long-term use, you can choose TAPS buffer powder. This is more conducive to long-term storage. It can be prepared at any time when it is used, and different concentrations and pH values can be adjusted according to needs, making it more flexible to use. Moreover, TAPS buffer powder can save a lot in price compared with ready-to-use buffers.
2. Quality selection
As an established manufacturer of in-vitro diagnostic reagents, Desheng Biochemical produces TAPS buffers with high purity and good quality. We have specially equipped professionals to test the various indicators of the products. The products have the characteristics of high purity and poor batches. The buffering performance is stable and has little effect on the experimental results.
3. Delivery speed
We all have ample supply of goods in stock, whether it is a large-volume or small-volume order, we can prepare the goods very quickly. In addition, our TAPS powder reagents are also faster and safer. Unlike ready-to-use solutions, there are requirements for packaging strength and tightness, so as to avoid crushing denaturation or breakage and leakage, and will not affect the experiment and procurement process.
4. After-sales issues
Desheng has always paid special attention to the after-sales problems of products. Good after-sales is a key factor to guarantee customers' subsequent repurchase, so we are equipped with 2 professional after-sales personnel to answer and solve the problems encountered by customers.