Wuhan Desheng Biochemical Technology Co., Ltd

products

Home /

products

Difference between CAPS and Tris in electrophoresis experiment White powder

Name: Difference between CAPS and Tris in electrophoresis experiment White powder
Brand: DESHENG
SKU: DESHENG
Rating: 5 (176 reviews)

Product Details

Place of Origin: EZHOU,CHINA

Brand Name: DESHENG

Certification: ISO9001:2008

Payment & Shipping Terms

Minimum Order Quantity: 10g

Price: Negotiable

Packaging Details: Plastic Bottle or Aluminium Film

Delivery Time: 1~3 DAYS AFTER RECEIVING PAYMENT

Payment Terms: T/T, L/C, D/A, D/P, Western Union, MoneyGram,paypal

Supply Ability: 100kg/month

Get Best Price

Contact Now

Specifications

Description

Specifications

Highlight: 
                                                                                                                                                                                                                                                                                                                                                                                                                                    3 morpholinopropanesulfonic acid, CAPS buffer, 1135-40-6
                                                      

Appearance:	White Crystal Powder	Purity:	>99.0%
MW:	221.32	Formula:	C9H19NO3S
Cas:	1135-40-6	Name:	CAPS

Appearance:	White Crystal Powder
Purity:	>99.0%
MW:	221.32
Formula:	C9H19NO3S
Cas:	1135-40-6
Name:	CAPS

Description

Difference between CAPS and Tris in electrophoresis experiment White powder

Product Name: N-Cyclohexyl-3-aminopropanesulfonic acid

Chemical Name:CAPS buffer

CAS No.: 1135-40-6

CAPS reagent, namely cyclohexamine propanesulfonic acid, is an aminosulfonate with amphoteric properties. The most widely used reagent is in water-borne polyisocyanate coatings, as well as in biological buffers for biochemical experiments such as HPLC separation and SDS-PAGE gel electrophoresis.

Difference between CAPS and Tris in electrophoresis experiment White powder 0

CAPS powder

Chinese Name	3- cyclohexylamine) -1- propyl sulfonic acid
English Names	N-Cyclohexyl-3-aminopropanesulfonic acid
CAS No	1135-40-6
Appearance	White crystal powder
Purity	>99%
Molecular formula	C9H19NO3S
Molecular weight	221.32
Packing	25kg per drum

As a biological buffer, CAPS, similar to Tris and MOPS, can be used in electrophoresis buffer, but there are differences. We can understand the requirements of electrophoresis buffer first.Electrophoresis refers to the movement of charged particles under the action of electric field, thinking of the electrode opposite to its electrical property, under certain conditions, the movement rate (mobility) of charged particles is fixed.In biochemical experiments, charged particles are usually protein colloids, nucleic acids, etc., and different proteins or nucleic acids have different mobility, thus separating each other.Since proteins and nucleic acid particles are very sensitive to pH, buffers must be added to the electrophoresis solution to protect proteins or nucleic acids.

When the conditions such as voltage fixation and electrophoresis time of the applied electric field are the same, the mobility of different electrophoretic particles is different, so the mobility of proteins or nucleic acids is different with different sizes or types.The difference in mobility of different particles, i.e. the separation distance, is proportional to voltage and electrophoresis time.Depending on the size of the electrophoretic particles, the electrophoretic buffers used are also different.

CAPS buffer: It is suitable for the electrophoresis of high molecular weight proteins (greater than 20 KD), i.e. western blot.Since only EDTA and methanol are contained in the electrophoretic buffer prepared by CAPS, PVDF for protein gel electrophoresis can be used not only for protein separation, but also for subsequent protein sequencing.

Tris buffer: It is usually used for electrophoresis of nucleic acid DNA, i.e. Southern blot.Among them, TEA is used for DNA fragments larger than 13 KB with good separation effect and can be used to recover DNA; TBA is used for DNA fragments smaller than 1 kb, but boric acid in it will affect DNA recovery and subsequent enzymatic reaction experiments.Tris can also be used for imprinting experiments of low molecular weight proteins, but glycine can not be used for protein sequencing, and Tris-Tricine + EDTA buffer system is needed.

MOPS buffer: It is usually used to prepare RNA electrophoresis buffer, i.e. Northern blot, when electrophoresis.

In addition to the CAPS, Tris and MOPS mentioned above, there are some other electrophoretic buffers. In the whole electroblot experiment, the cost of the buffer is less than that of nucleic acid dyes, proteins, etc. It is suggested to use high-quality buffer raw materials to ensure the experimental effect. Desheng specializes in the development and production of various biological buffers, which can meet the buffer requirements of different experiments.

Product name	Purity	Physical properties	Absorbance
Bicine	＞99%	White crystal powder	＜0.05
TAPS	＞99%	White crystal powder	＜0.05
CAPS	＞99%	White crystal powder	＜0.05
EPPS	＞99%	White crystal powder	＜0.1
MOPS	＞99%	White crystal powder	＜0.05
The above are our independently developed GOOD ’S buffer products. Our advantages are: high product purity, stable process, absorbance <0.05 (this index is more important), and low cost.

Difference between CAPS and Tris in electrophoresis experiment White powder 1

Product packaging

As a manufacturer of biological buffering agents such as CAPS, Desheng not only provides analytical grade raw materials, but also serves as a manufacturer for beginners to purchase. With complete qualifications, it is suitable for various biochemical experiments. If you have any needs, please feel free to click on the website to inquire about details!

Tags:

Please send us your request and we will reply to you as soon as possible.

Send

Blood Collection Tube Additives

Chemiluminescent Reagent

Good Buffer Solutions

Tromethamine

Trinder Reagent

Biological Buffer

Enzyme Preparation

Antigen Antibody

Serum Separator Gel

Anticoagulant For Blood Collection

Blood Clot Activator

Blood Coagulant Powder

Intermediate

Difference between CAPS and Tris in electrophoresis experiment White powder

3 morpholinopropanesulfonic acid

CAPS buffer

1135-40-6

Good's Buffers,

Biological buffer,

Tris(Hydroxymethyl)Aminomethane

Good Buffer Solutions BICINE CAS150-25-4 white crystalline powderPurity>99%

Good Buffer Solutions HEPES CAS7365-45-9 Ethanesulfonic Acid Purity>99%

Biological buffer TAPS buffer solutions ，CAS29915-38-6，Purity > 99%

Good Buffer Solutions CAPS High Purity> 99% White crystal powder CAS1135-40-6

Good Buffer Solutions MOPS of reagent company for blood testing CAS1132-61-2

Good Buffer Solutions PIPES CAS5625-37-6 White crystal powder Purity>99%

CAS 35556-70-8 High Purity White Biological Buffer PEP Powder

Characteristics and Application of Good's Buffer MOPS CAS 1132-61-2 White Purity

Good Buffer Solution CAPS white crystal powder CAS1135-40-6

Synthesis of Biological Buffer TAPS CAS29915-38-6 White Powder with High Purity

Good Buffer Solutions MOPSO CAS 68399-77-9 with high purity>99%

Blood Collection Tube Additives

Chemiluminescent Reagent

Good Buffer Solutions

Tromethamine

Trinder Reagent

Biological Buffer