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Difference between CAPS and Tris in electrophoresis experiment White powder

Name: Difference between CAPS and Tris in electrophoresis experiment White powder
Brand: DESHENG
SKU: DESHENG
Rating: 4.9 (186 reviews)

Product Details

Place of Origin: EZHOU,CHINA

Brand Name: DESHENG

Certification: ISO9001:2008

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Minimum Order Quantity: 10g

Price: Negotiable

Packaging Details: Plastic Bottle or Aluminium Film

Delivery Time: 1~3 DAYS AFTER RECEIVING PAYMENT

Payment Terms: T/T, L/C, D/A, D/P, Western Union, MoneyGram,paypal

Supply Ability: 100kg/month

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Specifications

Description

Specifications

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                                                                                                                                                                                                                                                                                                                                                                                                                                    3 morpholinopropanesulfonic acid, medical laboratory reagents
                                                      

Appearance:	White Crystal Powder	Purity:	>99.0%
MW:	221.32	Formula:	C9H19NO3S
Cas:	1135-40-6	Name:	CAPS

Appearance:	White Crystal Powder
Purity:	>99.0%
MW:	221.32
Formula:	C9H19NO3S
Cas:	1135-40-6
Name:	CAPS

Description

Difference between CAPS and Tris in electrophoresis experiment White powder

Product Name: N-Cyclohexyl-3-aminopropanesulfonic acid

Chemical Name:CAPS

CAS No.: 1135-40-6

CAPS reagent, namely cyclohexamine propanesulfonic acid, is an aminosulfonate with amphoteric properties. The most widely used reagent is in water-borne polyisocyanate coatings, as well as in biological buffers for biochemical experiments such as HPLC separation and SDS-PAGE gel electrophoresis.

Chinese Name	3- cyclohexylamine) -1- propyl sulfonic acid
English Names	N-Cyclohexyl-3-aminopropanesulfonic acid
CAS No	1135-40-6
Appearance	White crystal powder
Purity	>99%
Molecular formula	C9H19NO3S
Molecular weight	221.32
Packing	25kg per drum

As a biological buffer, CAPS, similar to Tris and MOPS, can be used in electrophoresis buffer, but there are differences. We can understand the requirements of electrophoresis buffer first.Electrophoresis refers to the movement of charged particles under the action of electric field, thinking of the electrode opposite to its electrical property, under certain conditions, the movement rate (mobility) of charged particles is fixed.In biochemical experiments, charged particles are usually protein colloids, nucleic acids, etc., and different proteins or nucleic acids have different mobility, thus separating each other.Since proteins and nucleic acid particles are very sensitive to pH, buffers must be added to the electrophoresis solution to protect proteins or nucleic acids.

CAPS

When the conditions such as voltage fixation and electrophoresis time of the applied electric field are the same, the mobility of different electrophoretic particles is different, so the mobility of proteins or nucleic acids is different with different sizes or types.The difference in mobility of different particles, i.e. the separation distance, is proportional to voltage and electrophoresis time.Depending on the size of the electrophoretic particles, the electrophoretic buffers used are also different.

CAPS buffer: It is suitable for the electrophoresis of high molecular weight proteins (greater than 20 KD), i.e. western blot.Since only EDTA and methanol are contained in the electrophoretic buffer prepared by CAPS, PVDF for protein gel electrophoresis can be used not only for protein separation, but also for subsequent protein sequencing.

Tris buffer: It is usually used for electrophoresis of nucleic acid DNA, i.e. Southern blot.Among them, TEA is used for DNA fragments larger than 13 KB with good separation effect and can be used to recover DNA; TBA is used for DNA fragments smaller than 1 kb, but boric acid in it will affect DNA recovery and subsequent enzymatic reaction experiments.Tris can also be used for imprinting experiments of low molecular weight proteins, but glycine can not be used for protein sequencing, and Tris-Tricine + EDTA buffer system is needed.

MOPS buffer: It is usually used to prepare RNA electrophoresis buffer, i.e. Northern blot, when electrophoresis.

In addition to the CAPS, Tris and MOPS mentioned above, there are some other electrophoretic buffers. In the whole electroblot experiment, the cost of the buffer is less than that of nucleic acid dyes, proteins, etc. It is suggested to use high-quality buffer raw materials to ensure the experimental effect. Desheng specializes in the development and production of various biological buffers, which can meet the buffer requirements of different experiments.

Product name	Purity	Physical properties	Absorbance
Bicine	＞99%	White crystal powder	＜0.05
TAPS	＞99%	White crystal powder	＜0.05
CAPS	＞99%	White crystal powder	＜0.05
EPPS	＞99%	White crystal powder	＜0.1
MOPS	＞99%	White crystal powder	＜0.05
The above are our independently developed GOOD ’S buffer products. Our advantages are: high product purity, stable process, absorbance <0.05 (this index is more important), and low cost.

Difference between CAPS and Tris in electrophoresis experiment White powder 2

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