Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 500g
Price: Consult the latest price
Packaging Details: 500g / bottle, 25kg / barrel
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 1000kg/a week
Brand: |
Desheng |
Purity: |
＞99% |
Molecular Formula: |
C7H17NO6S |
Molecular Weight: |
243.28 |
EINECS: |
249-954-1 |
Mol: |
29915-38-6.mol |
Brand: |
Desheng |
Purity: |
＞99% |
Molecular Formula: |
C7H17NO6S |
Molecular Weight: |
243.28 |
EINECS: |
249-954-1 |
Mol: |
29915-38-6.mol |
Application of n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid biological buffer,CAS29915-38-6 supplier
Taps, n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, is a member of the good's buffer family. It is a kind of amphoteric buffer widely used in biochemistry and molecular biology. It is often used as a buffer for the separation of dyes in planar chromatography and for the purification of enzymes in ion exchange chromatography.
| appearance | White powder |
| nature | Soluble in water |
| PH buffer range | 7.7-9.1 |
| pKa(25℃) | 8.40 |
1, Biological buffer for the separation of dyes in chromatography
Picture: taps is used for chromatographic separation of dyes
Chromatography is a laboratory technique, usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Since the retention of ionizable compounds is particularly sensitive to the pH of the mobile phase, a buffer should be added to the system to control this variable.
Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers including taps have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.
2, One of the most suitable biological buffers for cell culture
Picture: taps for cell culture
As mentioned above, taps is a member of the good's family of buffers. One of the most important features of good's buffers is that they are non-toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH value under control. Due to their special functions, many good's Buffers / biological buffers are considered ideal for cell isolation, cell culture, enzyme analysis and many other biochemical applications.
Desheng's researchers found that protonated sulfamate compounds directly inhibit the activity of connexin channels. In good's pH buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] - 1), pH dependent connexin channel activity demonstrated this. -Propane sulfonic acid), which share a common sulfamate moiety and have no pH dependent channel activity in pH buffer without sulfamate moiety. Therefore, taps is more used for flagellate algae experiment in cell culture medium.
Hubei xindesheng Material Technology Co., Ltd. is committed to the R & D, production and sales of biological buffers. Can provide pure white appearance products, products with high purity, small batch difference and other characteristics. Welcome to contact us www.whdsbio.com.
