99.9% Chemiluminescence Analysis Characteristics Of Luminol And Acridinium Esters

99.9% Chemiluminescence analysis characteristics of luminol and acridinium esters

Chemiluminescence analysis characteristics of luminol and acridinium esters
Acridine esters and luminol are both chemiluminescence reagents. In addition to their different sensitivity, there is also a big difference in chemiluminescence immunoassay combined with antigen-antibody immunoreactions. Luminol requires enzyme catalysis. Acridine esters chemiluminescence No enzymes are required.

Direct chemiluminescence of luminol
If it is pure luminol chemiluminescence analysis, enzyme catalysis is not needed. For example, when luminol detects trace blood stains, only luminol reagent and alkaline hydrogen peroxide solution are needed, because the blood stains contain iron porphyrin (Hemoglobin) has a catalytic effect; other oxidizing substances such as iron, copper and other metal ions or hypochlorite ions can also catalyze the oxidation of luminol or directly oxidize luminol to emit light, so luminol can also be used For the detection of such substances.

Chemiluminescence of Luminol and Acridinium Ester

Enzymatic chemiluminescence of luminol
Luminol's chemiluminescence sensitivity is very high, but when used in the detection of more trace substances such as immunoassays, this requires peroxidase to catalyze the luminescence of luminol. The enzymatic chemiluminescence of luminol is similar to the enzyme-labeled ELISA. Enzyme is used to label the antigen or antibody, except that luminol is used as the luminescent substrate for the enzyme reaction in the last step of the reaction (the chromogenic substrate in ELISA).
Direct chemiluminescence of acridine esters

The chemiluminescence detection sensitivity of acridinium ester is higher than that of luminol, and it does not require enzyme catalysis, which is suitable for the detection of very small amounts of substances. Acridine esters can be directly used as labels for antigens, antibodies and even nucleic acids. The addition of hydrogen peroxide under alkaline conditions can directly stimulate chemiluminescence reactions, and the luminescence reactions are not affected by the labeled protein or antibody. This is because during the chemiluminescence reaction, the labeled protein or nucleic acid will be used as a leaving group from the acridinium ester parent.

In summary, luminol can enzymatic chemiluminescence can also be direct chemiluminescence, acridinium ester is direct chemiluminescence, the main difference in detection lies in the detection limit, that is, the sensitivity. Desheng has its own technical research and development team, which has unique advantages in the development and production of chemiluminescence reagents.