Cas 7365-45-9 EINECS 230-907-9 HEPES Buffer For Lymphocyte Culture

HEPES buffer for lymphocyte culture cas 7365-45-9 EINECS 230-907-9

HEPES buffer for lymphocyte culture

In the process of cell culture, cell metabolism will affect the drastic changes in the pH value of the culture medium environment. Therefore, in order to maintain a suitable culture environment for the cells, it is necessary to add a biological buffer to adjust the pH value. There are many types of biological buffers, and there are many that can be used for cell culture, but the most suitable one is HEPES buffer. Compared with other buffers, its pH range is pH 6.8-pH 8.2, and the most favorable environment pH for cell culture is pH 7.2-pH 7.4, so it falls within this range, which can bring more to cell culture. Stable environment.

Biological buffer HEPES
Due to the better performance of HEPES in cell culture media, research has found that it is also suitable for use as a buffer in human peripheral blood lymphocyte cultures, and has the following advantages:
1. It is non-toxic to lymphocytes in the concentration range (10-40 m M) and has excellent buffering capacity.
2. It does not stimulate lymphocytes by itself.
3. It basically does not affect the lymphocyte stimulation rate induced by non-specific stimulants (phytohemagglutinin), antigens (tuberculin) or mixed allogeneic cultures.

Compared with another bicarbonate buffer commonly used in cell culture, it provides greater buffering capacity in the physiological range of pH. Therefore, even in very crowded cultures, HEPES buffered medium can maintain a constant pH value, ensuring better cell survival and stimulation. PHA-induced lymphocytes were observed to produce great stimulation in HEPES buffered cultures in the pH range of 7.4-7.6.

The above briefly describes the advantages of HEPES in the field of cell culture, which is why HEPES is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological and biochemical research. Desheng Company is a company specializing in the production of biological buffers. It has been engaged in the research and development and production of HEPES for more than ten years. The product knowledge is very rich. Please feel free to contact us if you need it!