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|Molecular Weight:||163.17||Molecular Formula:||C6H13NO4|
|Apperance:||White Crystal Powxer||Purity:||>99%|
Cas #150-25-4 Good's Buffer,
Bicine Good's Buffer,
Cas #150-25-4 99% Bicine
BUFFER SOLUTIONS;GOOD'S BUFFER, BICINE; Cas #150-25-4 WHITE CRYSTAL POWDER PURITY>99%
Introduction and preparation method of biological buffer BICINE
Label: BICINE, preparation
Introduction: Buffer is a solution that can resist pH changes when a small amount of acid or alkali and water are added. PH buffer system plays an important role in maintaining the normal pH value of organisms and the normal physiological environment. Most cells can only work at a narrow pH Activities are carried out within the scope, and a buffer system is required to resist pH changes during the metabolic process. There are three main pH buffer systems in organisms, which are protein and bicarbonate buffer systems. Each buffer system accounts for The amount of is different in various types of cells and organs.
|CAS No||150-25-4||Molecular formula||C6H13NO4|
|Appearance||White crystal powder||Purity||≥99%|
|Storage Conditions||Room temperature, light-proof and moisture-proof|
In biochemical research work, buffer solutions are often used to maintain the pH of the experimental system. Changes in the pH value of the solution system for research work often directly affect the effectiveness of our work. If the pH value of the extraction enzyme experimental system changes or changes too much , It will reduce the enzyme activity or even completely inactivate. So we have to learn to prepare buffer.
Bicine is one of the commonly used biological buffers. It is an amphoteric amino acid buffer and is active in the pH range of 7.6-9.0 (pKa of 8.3 at 25°C). Recommended buffer for low temperature biochemical work. Bicaine is used to prepare a stable serum guanosine enzyme substrate solution. A method for protein separation using dihydropyridine in thin-layer ion exchange chromatography has been published. Dihydropyridine has been used for peptide and protein crystallization.
Finished buffer standard
Preparation method of Bicine solution (about 1-2L):
(1) With 0.1M solution (A): Bicine 16.317g/ deionized water 1,000ml
(2) With 0.1M NaOH solution (B): NaOH 4g/ deionized water 1,000ml
(3) pH 5.1 1,000ml(A)+0ml(B) (A):(B)=5:0
pH 7.8 1,000ml(A)+200ml(B) (A):(B)=5:1
pH 8.2 1,000ml(A)+400ml(B) (A):(B)=5:2
pH 8.6 1,000ml(A)+600ml(B) (A):(B)=5:3
pH 10.4 1,000ml(A)+800ml(B) (A):(B)=5:4
*The temperature is 20 degrees.
*If you need to adjust to a specific pH, please use a pH meter.
*Don't want to add Na, please use KOH.
Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of various biological buffers, including: Tris, Tris HCl, BICINE, CAPS, MOPS, TAPS, EPPS, MOPSO, HEPES, POPES, please inquire if you need it.
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