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|CAS NO:||7365-45-9||EINECS NO:||230-907-9|
|Merck :||14,4654||BRN :||883043|
powdered biological buffer HEPES,
4-Hydroxyethyl Piperazine Ethanesulfonic Acid
Application of biological buffer 4-hydroxyethyl piperazine ethanesulfonic acid in kit
The chemical name of biological buffer HEPES is 4-hydroxyethyl piperazine ethanesulfonic acid, which is white crystalline powder. It is a kind of weak acid and amphoteric buffer in organic chemical industry.
The decomposition ability of 4-hydroxyethyl piperazine ethanesulfonic acid increased with the increase of temperature and decreased with the decrease of temperature. However, compared with other buffers, the decomposition constant of 4-hydroxyethyl piperazine ethanesulfonic acid changed little with temperature, which made HEPES buffer a buffer that could maintain the structure and function of enzyme at low temperature. It is widely used in clinical laboratory diagnostic reagents, life science research, pharmaceutical and biotechnology and many other fields.
|The pH range of acid-base indicator||7.0-7.6|
|Acidity coefficient (PKA)||7.5 chemical book (at 25 ℃)|
|Maximum wavelength (λ max) λ||260 nmAmax:0.06 λ:280 nmAmax:0.04|
1) Effect of biological buffer HEPES on the stability of foot and mouth disease antigen.
Foot and mouth disease antigen is easy to degrade in the process of storage and vaccine preparation, which affects the effect of vaccine. The degradation rate of foot-and-mouth disease antigen is related to the stability of pH value of maintenance solution. The preparation of maintenance solution with strong pH stability can improve the quality of foot-and-mouth disease vaccine.
2) Preparation of a skin care product containing biological buffer
The invention comprises a microbial fermentation product extract, which is characterized in that it also comprises a biological buffer with a mass percentage of 0.1% - 3.0%. Advantages: the pH value drift range of the product is small. Within three years after the preparation of skin care products, the pH value can be stable at 6.0-8.0, which has good protection for the microbial fermentation product extract added. The microbial fermentation product extract is not easy to be hydrolyzed, and the anti-aging effect of the product lasts for a long time.
3) Objective to prepare a kit for detecting the content of 25 hydroxyvitamin D3.
The kit has the advantages of high sensitivity, high specificity, accurate quantification and wide linear range. It is especially suitable for the detection of samples with very low content of 25 hydroxyvitamin D3. In addition, the kit of the invention does not need to pre dilute the sample in the detection process, is convenient for clinical use, has simple operation and low detection cost, and is suitable for various automatic biochemical analyzers.
4) Preparation of lactate dehydrogenase detection kit.
The detection kit adopts double reagent mode, which can effectively avoid the interference of non-specific reaction and minimize the interference from the turbidity of the sample itself, ensuring the stability and reliability of the determination results. It has the advantages of good stability, high accuracy, wide linear test range, good repeatability and strong anti-interference. In addition, the detection kit does not need to pre dilute the sample in the detection process, which is convenient for clinical use, simple and fast operation, and is suitable for automatic biochemical analyzer.
5) To prepare a neuron specific enolase content detection kit, which belongs to the field of human neuron specific enolase content detection.
The kit has the advantages of high sensitivity, wide linear range, simple operation, high specificity, good stability and high sensitivity.
In addition to HEPES, biological buffers produced by Desheng include Tris, bicine, caps, mops, taps, EPPs, etc., which are mainly used in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits. Customers who need can provide samples.
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