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|Purity:||＞99%||Chemical Name:||HEPES, 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid|
CAS 7365-45-9 HEPES,
99% hepes powder,
7365-45-9 hepes powder
HEPES (CAS 7365-45-9) ,2-(4-(2-Hydroxyethyl)Piperazin-1-Yl)Ethanesulfonic Acid,Purity＞99%, white powder
What is HEPES
HEPES as one of more than 20 kinds of good's reagents, HEPES is more used in cell culture, more broadly classified into biological buffers. The full name is 4-hydroxyethyl piperazine ethanesulfonic acid, also known as 2 - [4 - (hydroxyethyl) - 1-piperazinyl] ethanesulfonic acid, which is a zwitterionic sulfonic acid buffer. In 1966, Norman E. good proposed that HEPES is a good's buffer.
Buffer range of HEPES
Most buffers realize their own "value" through their own buffer range. The pH range of buffer capacity that can be exerted by the buffer as a solvent is the buffer range. The buffer range of HEPES is 6.8 ~ 8.2, and the pKa is 7.48 at 25 ℃. The buffer ratio is 0.1-1.0. If it exceeds the buffer range, it is generally regarded as losing the function of buffer.
The use of HEPES
HEPES is more commonly used in cell culture, in vitro fertilization and embryo culture buffer, but HEPES can be combined with metal ions, most of the metal ion research is carried out by using HEPES buffer, although Tris and phosphate can also be used for metal ion research, but based on the characteristics of these buffers, it is necessary to choose different directions There will be some differences.
Nowadays, more and more attention is paid to the protection of the environment all over the world, and the analysis and some biological research in the environment is the use of HEPES buffer, more industrial grade HEPES. As a binding buffer and eluent in cation exchange, it is also used for grinding in plant research and development. In addition, HEPES is electrophoretic buffer, which is also commonly used in electroporation.
HEPES has a lot of functions, which will not be repeated here. The development of HEPES will be further expanded in the future.
Preservation of HEPES
HEPES raw material is white powder after production, which is more soluble in water. Therefore, when it is still white powder, it should be stored at room temperature and avoid direct sunlight. When the solvent is prepared, there are certain requirements on the storage temperature. It should be stored at 4 ℃, and it is recommended to use it in a short time for better effect.
Due to different purposes, the configuration method of HEPES is also different. HEPES is directly used with NaOH, and the other is HEPES with different salts. According to the adjustment of different pH to get the final suitable solvent, the specific needs of the configuration method of friends can be private message.
|λmax||λ: 260 nm Amax: 0.06λ: 280 nm Amax: 0.04|
Desheng biochemical is a professional manufacturer of HEPES, with a daily output of 1t and a purity of more than 99%. It also has a professional R & D team, and special indicators can be customized. Good product quality and preferential prices can bring surprise to friends in need!
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