HEPPS or EPPS are the common names for the compound 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid. It is used as a buffering agent in biology and biochemistry. The pKa of HEPPS is 8.00. Research on mice with Alzheimers disease-like [[Amyloid beta ]] plaques has shown that the EPPS can cause the plaques to break up, reversing some of the symptoms in the mice.
HEPPS mainly applies inbiochemical research.HEPPS have many properties similar to HEPES. Because of the high buffer range, it is suitable for phosphorylation, especially if Tricine is not available.HEPPS can be used in Folin protein detection, but cannot be used in biuret detection.It can also be used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.
The pH buffer range of PIPES is 6.1-7.5, insoluble in water and soluble in NaOH aqueous solution.The PIPES is different from the buffer containing di(2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), which cannot form stable complexes with most metal ions, and is suitable for buffer in solution systems containing metal ions.Based on the results of previous studies, PIPES can be applied to purify tubulin using cellulose phosphate chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to crystallize transketolase from Escherichia coli as a buffer.In addition, because PIPES can form free radicals, it is not suitable for application in redox systems.In cation exchange chromatography, a low concentration of PIPES buffer should be used because PIPES has relatively large ionic strength and its pKa value is concentration dependent.
The pH buffer range of HEPES is 6.8-8.2, which is soluble in water and does not form stable complexes with metal ions. In most cases, it does not interfere with biochemical processes. HEPES is often used in cell culture media of various types of organisms. In protein research, PIPES is often used as a component and eluent of binding buffer in cation exchange chromatography. In DNA research, PIPES is used as calcium phosphate and DN.A buffer for precipitate formation system, AFM and buffer in electroporation experiments.In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for the determination of protein content by Lowry's method.In summary, both PIPES and HEPS belong to Good's buffer, neither of them can form stable complexes with metal ions and are suitable for solution systems containing metal ions.But there are also some differences between them. In terms of solubility, PIPES is insoluble in water, while HEPES has good water solubility; in terms of buffer range, PIPES is acidic to neutral, HEPES is neutral to alkaline, which is mainly determined by the structural differences between the two. PIPES has two sulfonic groups, and HEPES contains one sulfonic acid group and hydroxyl group.In addition, PIPES and HEPES have certain limitations in some system applications.Therefore, we need to consider the suitability of the experimental system and the differences in the properties of the two when choosing the above buffers.In this way, when you purchase this Good's buffer, will you be able to better identify the product you need.De Sheng will also give you an accurate positioning.