Good Buffer Solutions EPPS with high buffer range CAS16052-06-5 Purity> 99%

EPPS Basic information

Molecular Structure

EPPS Chemical Properties

Description

N-2-Hydroxyethylpiperazine-N'-3-propanesulfonic acid is a commonly used agent in the laboratory, and is generally used to prepare buffers.

It can not form stable complexes with most metal ions and is suitable for buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox system. Low concentration of PIPES buffer should be used in cation exchange chromatography because PIPES has relatively high ionic strength and its pKa value is concentration dependent.

EPPS(Powder form)

Application

1.EPPS has many characteristics similar to HEPES. Because of its high buffer range, it is suitable for phosphorylation reactions, especially when TriClne cannot be used.

2.It is used to detect Folin protein, enhance glucose phosphate mutase, as a separating agent in biological buffer, ultra-thin isoelectric focusing gel, etc.

Advantage

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

Good's buffer characteristics

Good's buffer is also known as zwitterionic buffer. In 1960, N.E.Good and his colleagues summarized the advantages and disadvantages of the existing buffer reagents, and believed that it was necessary to use artificial design and artificial synthesis methods to find specific buffer systems for life science research. The system should have the following characteristics:

1. The pKa value is between 6-8;

2. High solubility in water;

3. Not easy to penetrate biofilm;

4. Small salt effect;

5. The ion concentration, solution composition and temperature have little effect on dissociation;

6. No complex or precipitation with metal ions;

7. The buffer is chemically stable;

8. Small light absorption in the ultraviolet and visible light wavelength range;

9. Easily produce high-purity salt.

The main advantages of Good's buffer:

It does not participate in or interfere with the biochemical reaction process, and has no inhibitory effect on enzyme chemical reactions, etc., so they are specially used for the research work of organelles and highly volatile, pH-sensitive proteins and enzymes.

Hubei New Desheng Technology Co., Ltd. was founded in 2005, specializing in the research, development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. In terms of blood collection tube additives, it has formed its own intellectual property rights and professional production research and development capabilities. Provide products and raw material solutions for more than 100 domestic and foreign manufacturers.
At present, the Good buffer solutions supplied by our company include Tris, Bicine, CAPS, MOPS, TAPS, EPPS, etc .,all of which is white crystals, purity> 99%, absorbance <0.05. Welcome enterprises or individuals to call for details.