CAS 150-25-4 Good Buffer Solutions N,N-Bis(2-Hydroxyethyl)Glycine In IVD

Application of N,N-Bis(2-Hydroxyethyl)Glycine in IVD,CAS 150-25-4,Biological buffer supplier

Bicine (n, n-dihydroxyethylglycine, CAS: 150-25-4) is a kind of white powder, which is widely used as an organic compound of buffer. Bicine is a zwitterionic biological buffer, which is usually used as a buffer in biological and biochemical research.

It is one of the buffers of good's and its PKA is 8.35 at 20 ° C. It is prepared by the reaction of glycine with ethylene oxide and hydrolysis of the lactone. Bicine can be used to remove pollutants in amine system of gas desulfurization. It is formed by amine degradation in the presence of O 2, so 2, H 2S or thiosulfate.

Picture: bicine by Desheng

Picture: pH range of bicine and other buffers

1, Bicine as a good's buffer is usually used in protein crystallization solution

Bicine is a widely used buffer, which is used for enzyme reaction buffer and electrophoresis buffer. The working concentration ranges from 3 to 100 mm. It is used for biological buffer, especially for low temperature biochemical research, for preparation of stable substrate solution for determination of serum guaninase, and for separation of plasma proteins by isokinetic electrophoresis with cross-linked dextran.

2, Bicine has been used as a buffer in chromatography and polymerase chain reaction

Bicine is an amphoteric amino acid buffer with activity in the range of pH 7.6-9.0 (PKA = 8.3 at 25 ° C). It is the recommended buffer for low temperature biochemical reaction. Moreover, it has the advantages of not participating in or interfering with the biochemical reaction process, being resistant to chemical action and enzymatic hydrolysis, so it is often used as a buffer in chromatography and polymerase chain reaction.

3, Mobile phase buffer and eluent of bicine in cation exchange chromatography

The application of bicine in thin layer ion exchange has been confirmed for protein resolution chromatography. Bicine has been used for peptide and protein crystallization. Bicine was used in the reaction buffer. A protein SDS-PAGE multiphase buffer system combined with bicine peptide has been widely used in in vitro diagnostic clinical trials.

4, It can be used as chelating agent in soil remediation.

Because bicine contains two - oh and one - COOH, it can chelate copper, cadmium, lead and other heavy metal ions, but can not chelate calcium and magnesium ions. Therefore, bicine is often used as a chelating agent for remediation of heavy metal contaminated soil.

Bicine | 150-25-4 | has good elution ability for heavy metals under neutral and acidic conditions, and can effectively remove Cu, CD and Pb ions in contaminated soil. Bicine | 150-25-4 | is an active component of eluent, which can not only leach and repair heavy metal contaminated soil, but also avoid the loss of plant nutrient elements calcium and magnesium in soil, which is safe and environmentally friendly, and will not cause secondary pollution to the environment.

Desheng is a professional supplier of in vitro diagnostic reagents and vascular additive reagents, providing buffers such as caps, mops, Tris, MOPSO, HEPES and various anticoagulant and procoagulant reagents. The purity of buffer produced by our company is more than 99%, the absorbance is less than 0.05, the water solubility is good, and the process is stable, which can ensure that the appearance of the product is pure white crystal powder.